2018年4月
Andrographolide from the herb Andrographis paniculata induces apoptosis on cultured human leukemic cells
Fujita Med.J.
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- 巻
- 3
- 号
- 3
- 開始ページ
- 48
- 終了ページ
- 54
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.20407/fmj.3.3_48
- 出版者・発行元
- 藤田学園医学会
<p>Objectives: Andrographis paniculata (A. paniculata) is a widely used herb that has potential medical properties. Andrographolide (Andro) is the major component of A. paniculata. We evaluated the anti-tumor activity of Andro using leukemic cell line cells.</p><p>Methods: Leukemic cell lines U937, HL60 or H929 cells were cultured in the presence or absence of Andro and compared with the effects of Ara-C or vincristine. The anti-tumor activity was assessed by morphological observations of the cells, DNA fragmentation, MTT assay, Annexin V positive rate, caspase-3/7 activity, and cell cycle analysis.</p><p>Results: After addition of Andro, the morphology of cells changed to characteristic shapes with apoptotic bodies. Furthermore, the Annexin V positive rate and caspase-3/7 activities were increased compared with untreated cells. The G1 phase of cell cycle was also similarly increased compared with cells treated with Ara-C.</p><p>Conclusions: Our results show that Andro has an anti-tumor activity against leukemic cell lines, very possibly by inducing apoptosis.</p>
- リンク情報
- ID情報
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- DOI : 10.20407/fmj.3.3_48
- ISSN : 2189-7247
- CiNii Articles ID : 130006068163