MISC

2005年3月

Antiviral activity of human lactoferrin: Inhibition of alphavirus interaction with heparan sulfate

VIROLOGY
  • BL Waarts
  • ,
  • OJC Aneke
  • ,
  • JM Smit
  • ,
  • K Kimata
  • ,
  • R Bittman
  • ,
  • DKF Meijer
  • ,
  • J Wilschut

333
2
開始ページ
284
終了ページ
292
記述言語
英語
掲載種別
DOI
10.1016/j.virol.2005.01.010
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Human lactoferrin is a component of the non-specific immune system with distinct antiviral properties. We used alphaviruses, adapted to interaction with heparan sulfate (HS), as a tool to investigate the mechanism of lactoferrin's antiviral activity. Lactoferrin inhibited infection of BHK-21 cells by HS-adapted, but not by non-adapted, Sindbis virus (SIN) or Semliki Forest virus (SFV). Lactoferrin also inhibited binding of racholabeled HS-adapted viruses to BHK-21 cells or liposomes containing lipid-conjugated heparin as a receptor analog. On the other hand, low-pH-induced fusion of the viruses with liposomes, which occurs independently of virus-receptor interaction, was unaffected. Studies involving preincubation of virus or cells with lactoferrin suggested that the protein does not bind to the virus, but rather blocks HS-moieties on the cell surface. Charge-modified human serum albumin, with a net positive charge, had a similar antiviral effect against HS-adapted SIN and SFV, suggesting that the antiviral activity of lactoferrin is related to its positive charge. It is concluded that human lactoferrin inhibits viral infection by interfering with virus-receptor interaction rather than by affecting subsequent steps in the viral cell entry or replication processes. (C) 2005 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.virol.2005.01.010
CiNii Articles
http://ci.nii.ac.jp/naid/80017228587
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15721362
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000227450300009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.virol.2005.01.010
  • ISSN : 0042-6822
  • CiNii Articles ID : 80017228587
  • PubMed ID : 15721362
  • Web of Science ID : WOS:000227450300009

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