2010年7月
Enzymatic synthesis of caffeic acid phenethyl ester analogues in ionic liquid
JOURNAL OF BIOTECHNOLOGY
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- 巻
- 148
- 号
- 2-3
- 開始ページ
- 133
- 終了ページ
- 138
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jbiotec.2010.05.007
- 出版者・発行元
- ELSEVIER SCIENCE BV
An efficient procedure for transesterification of methyl caffeate was developed to produce caffeic acid phenethyl ester analogues with Candida antarctica lipase B using an ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide, as a solvent. The system provided 48.8 mM 2-cyclohexylethyl caffeate and 46.9 mM 3-cyclohexylpropyl caffeate with conversion yields of 97.6% and 93.8%, respectively. Reusability of the system was investigated, and the yield of 4-phenylbutyl caffeate was increased from 30.4 to 45.7 mM when the transesterification was carried out under reduced pressure to remove a by-product, methanol. Additionally, we showed that both 2-cyclohexylethyl caffeate and 3-cyclohexylpropyl caffeate exhibit strong antiproliferative activities, which are comparable to that of 5-fluorouracil by MTT assay. (C) 2010 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.jbiotec.2010.05.007
- ISSN : 0168-1656
- Web of Science ID : WOS:000280395100007