2006年11月
Identification and functional analysis of bifunctional ent-kaurene synthase from the moss Physcomitrella patens
FEBS LETTERS
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- 巻
- 580
- 号
- 26
- 開始ページ
- 6175
- 終了ページ
- 6181
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.febslet.2006.10.018
- 出版者・発行元
- ELSEVIER SCIENCE BV
ent-Kaurene is the key intermediate in biosynthesis of gibberellins; (GAs), plant hormones. In higher plants, ent-kaurene is svnthesized successively by copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) from geranylgeranyl diphosphate (GGDP). On the other hand, fungal ent-kaurene synthases are bifunctional cyclases with both CPS and KS activity in a single polypeptide. The moss Physcomitrella patens is a model organism for the study of genetics and development in an early land plant. We identified ent-kaurene synthase (PpCPS/KS) from P. patens and analyzed its function. PpCPS/KS cDNA encodes a 101-kDa polypeptide, and shows high similarity with CPSs and abietadiene synthase from higher plants. PpCPS/KS is a bifunctional cyclase and, like fungal CPS/KS, directly synthesizes the ent-kaurene skeleton from GGDP. PpCPS/KS has two aspartate-rich DVDD and DDYFD motifs observed in CPS and KS, respectively. The mutational analysis of two conserved motifs in PpCPS/KS indicated that the DVDD motif is responsible for CPS activity (GGDP to CDP) and the DDYFD motif for KS activity (CDP to ent-kaurene and ent-16 alpha-hydroxy-kaurene). (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.febslet.2006.10.018
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018824044
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17064690
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000242233100023&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.febslet.2006.10.018
- ISSN : 0014-5793
- CiNii Articles ID : 80018824044
- PubMed ID : 17064690
- Web of Science ID : WOS:000242233100023