論文

査読有り
2015年7月

Immunohistological analysis for immunological response and mechanism of interstitial fibrosis in IgG4-related kidney disease

MODERN RHEUMATOLOGY
  • Eiichi Kawamura
  • ,
  • Satoshi Hisano
  • ,
  • Hitoshi Nakashima
  • ,
  • Morishige Takeshita
  • ,
  • Takao Saito

25
4
開始ページ
571
終了ページ
578
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3109/14397595.2014.1001474
出版者・発行元
SPRINGER

Objectives. Our study aimed to clarify the immunological characteristics and the mechanism of interstitial fibrosis in immunoglobulin G4-related kidney disease (IgG4-RKD) by the immunohistological analysis.
Methods. Immunohistological study was performed in the biopsied renal tissues of 16 IgG4-RKD, 16 Sjogren syndrome (SJS), and 17 idiopathic tubulointerstitial nephritis (ITIN) patients using antibodies against IgG; IgG1; IgG4; CD38; CD3; C-X-C chemokine receptor type 3 (CXCR3); chemokine (C-C motif) receptor 4 (CCR4); forkhead box 3 (Foxp3); Type I, Type III, Type IV, and Type VI collagens; and transforming growth factor (TGF)-beta 1.
Results. Interstitial lymphoplasmacytic and eosinophilic infiltration and the severity of interstitial fibrosis were greater in IgG4-RKD than SJS and ITIN. The ratio of CXCR3+/CD3 + cells was greater in SJS as compared with that in IgG4-RKD and ITIN. The ratio of CCR4+/CD3 + cells was not different among the three diseases. The ratio of interstitial IgG4+/IgG + plasma cells, Foxp3+/CD3 + cells, and TGF-beta 1 + cells/total infiltrating cells was higher in IgG4-RKD than SJS and ITIN. There was a positive correlation between the ratio of Foxp3+/CD3 + cells and that of IgG4+/IgG+ plasma cells in IgG4-RKD. Significant correlation was found between the ratio of Foxp3+/CD3 + cells and that of TGF-beta 1 + cells/total infiltrating cells in IgG4-RKD. Foxp3 + cells and TGF-beta 1 + cells were colocalized in the interstitium in IgG4-RKD. The significant correlation between the ratio of TGF-beta 1 + cells/total infiltrating cells and the severity of fibrosis was noticed in IgG4-RKD. The interstitial distribution of type III collagen and type IV collagen was higher in IgG4-RKD than in SJS.
Conclusions. Our results suggest that regulatory T-cells (Tregs) may play a central role in IgG4 production in the interstitium and TGF-beta 1 induced by Tregs may play a pivotal role in the interstitial fibrosis including type III and type IV collagens in IgG4-RKD.

Web of Science ® 被引用回数 : 15

リンク情報
DOI
https://doi.org/10.3109/14397595.2014.1001474
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000358935600012&DestApp=WOS_CPL

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