論文

査読有り
2014年4月

Role of Retinoic Acid and Fibroblast Growth Factor 2 in Neural Differentiation from Cynomolgus Monkey (Macaca fascicularis) Embryonic Stem Cells

COMPARATIVE MEDICINE
  • Masanori Hatori
  • ,
  • Nobuhiro Shimozawa
  • ,
  • Lubna Yasmin
  • ,
  • Hirofumi Suemori
  • ,
  • Norio Nakatsuji
  • ,
  • Atsuo Ogura
  • ,
  • Ken-ichi Yagami
  • ,
  • Tadashi Sankai

64
2
開始ページ
140
終了ページ
147
記述言語
英語
掲載種別
研究論文(学術雑誌)
出版者・発行元
AMER ASSOC LABORATORY ANIMAL SCIENCE

Retinoic acid is a widely used factor in both mouse and human embryonic stem cells. It suppresses differentiation to mesoderm and enhances differentiation to ectoderm. Fibroblast growth factor 2 (FGF2) is widely used to induce differentiation to neurons in mice, yet in primates, including humans, it maintains embryonic stem cells in the undifferentiated state. In this study, we established an FGF2 low-dose-dependent embryonic stem cell line from cynomolgus monkeys and then analyzed neural differentiation in cultures supplemented with retinoic acid and FGF2. When only retinoic acid was added to culture, neurons differentiated from FGF2 low-dose-dependent embryonic stem cells. When both retinoic acid and FGF2 were added, neurons and astrocytes differentiated from the same embryonic stem cell line. Thus, retinoic acid promotes the differentiation from embryonic stem cells to neuroectoderm. Although FGF2 seems to promote self-renewal in stem cells, its effects on the differentiation of stem cells are influenced by the presence or absence of supplemental retinoic acid.

リンク情報
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000333722800010&DestApp=WOS_CPL
ID情報
  • ISSN : 1532-0820
  • Web of Science ID : WOS:000333722800010

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