2005年12月
1,25-Dihydroxyvitamin D-3 suppresses gene expression of eukaryotic translation initiation factor 2 in human promyelocytic leukemia HL-60 cells
CELL STRUCTURE AND FUNCTION
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- 巻
- 30
- 号
- 1-2
- 開始ページ
- 1
- 終了ページ
- 6
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1247/csf.30.1
- 出版者・発行元
- JAPAN SOC CELL BIOLOGY
The physiologically active metabolite of vitamin D-3, 1 alpha,25-dihydroxyvitamin D-3 (DVD), is a potent inducer of cell differentiation in human myeloid leukemia cells. In the present study, we examined changes in gene expression during DVD-induced cell differentiation of promyelocytic HL-60 cells employing a DNA microarray technique. The results identified 7 up-regulated and 9 down-regulated genes with a change greater than 1.5-fold after the DVD-treatment for both 2 and 6 days. Seven of these genes were further examined by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that findings obtained from the DNA microarray analysis and RT-PCR are generally comparable with each other. Gene expression of the subunits of eukaryotic translation initiation factor 2 was then examined by methods including RT-PCR and real-time PCR. The results indicated the suppression of these genes, suggesting a linkage to differentiation-associated growth inhibition of these cells.
- リンク情報
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- DOI
- https://doi.org/10.1247/csf.30.1
- CiNii Articles
- http://ci.nii.ac.jp/naid/40007227354
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15951637
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000237357200001&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1247/csf.30.1
- ISSN : 0386-7196
- eISSN : 1347-3700
- CiNii Articles ID : 40007227354
- PubMed ID : 15951637
- Web of Science ID : WOS:000237357200001