論文

査読有り
2014年5月

Identification of novel targets for antiangiogenic therapy by comparing the gene expressions of tumor and normal endothelial cells

Cancer Science
  • Tsuguteru Otsubo
  • ,
  • Yasuhiro Hida
  • ,
  • Noritaka Ohga
  • ,
  • Hideshi Sato
  • ,
  • Toshihiro Kai
  • ,
  • Yasushi Matsuki
  • ,
  • Hideo Takasu
  • ,
  • Kosuke Akiyama
  • ,
  • Nako Maishi
  • ,
  • Taisuke Kawamoto
  • ,
  • Nobuo Shinohara
  • ,
  • Katsuya Nonomura
  • ,
  • Kyoko Hida

105
5
開始ページ
560
終了ページ
567
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/cas.12394

Targeting tumor angiogenesis is an established strategy for cancer therapy. Because angiogenesis is not limited to pathological conditions such as cancer, molecular markers that can distinguish between physiological and pathological angiogenesis are required to develop more effective and safer approaches for cancer treatment. To identify such molecules, we determined the gene expression profiles of murine tumor endothelial cells (mTEC) and murine normal endothelial cells using DNA microarray analysis followed by quantitative reverse transcription-polymerase chain reaction analysis. We identified 131 genes that were differentially upregulated in mTEC. Functional analysis using siRNA-mediated gene silencing revealed five novel tumor endothelial cell markers that were involved in the proliferation or migration of mTEC. The expression of DEF6 and TMEM176B was upregulated in tumor vessels of human renal cell carcinoma specimens, suggesting that they are potential targets for antiangiogenic intervention for renal cell carcinoma. Comparative gene expression analysis revealed molecular differences between tumor endothelial cells and normal endothelial cells and identified novel tumor endothelial cell markers that may be exploited to target tumor angiogenesis for cancer treatment. © 2014 The Authors.

リンク情報
DOI
https://doi.org/10.1111/cas.12394
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24602018
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84899907702&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84899907702&origin=inward

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