論文

査読有り
2013年12月

The HSP90 Inhibitor Ganetespib Synergizes with the MET Kinase Inhibitor Crizotinib in both Crizotinib-Sensitive and -Resistant MET-Driven Tumor Models

CANCER RESEARCH
  • Naoto Miyajima
  • ,
  • Shinji Tsutsumi
  • ,
  • Carole Sourbier
  • ,
  • Kristin Beebe
  • ,
  • Mehdi Mollapour
  • ,
  • Candy Rivas
  • ,
  • Soichiro Yoshida
  • ,
  • Jane B. Trepel
  • ,
  • Ying Huang
  • ,
  • Manabu Tatokoro
  • ,
  • Nobuo Shinohara
  • ,
  • Katsuya Nonomura
  • ,
  • Len Neckers

73
23
開始ページ
7022
終了ページ
7033
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1158/0008-5472.CAN-13-1156
出版者・発行元
AMER ASSOC CANCER RESEARCH

The proto-oncogene MET is aberrantly activated via overexpression or mutation in numerous cancers, making it a prime anticancer molecular target. However, the clinical success of MET-directed tyrosine kinase inhibitors (TKI) has been limited due, in part, to mutations in the MET kinase domain that confer therapeutic resistance. Circumventing this problem remains a key challenge to improving durable responses in patients receiving MET-targeted therapy. MET is an HSP90-dependent kinase, and in this report we show that HSP90 preferentially interacts with and stabilizes activated MET, regardless of whether the activation is ligand-dependent or is a consequence of kinase domain mutation. In contrast, many MET-TKI show a preference for the inactive form of the kinase, and activating mutations in MET can confer resistance. Combining the HSP90 inhibitor ganetespib with the MET-TKI crizotinib achieves synergistic inhibition of MET, its downstream signaling pathways, and tumor growth in both TKI-sensitive and -resistant MET-driven tumor models. These data suggest that inclusion of an HSP90 inhibitor can partially restore TKI sensitivity to previously resistant MET mutants, and they provide the foundation for clinical evaluation of this therapeutic combination in patients with MET-driven cancers. (C)2013 AACR.

Web of Science ® 被引用回数 : 36

リンク情報
DOI
https://doi.org/10.1158/0008-5472.CAN-13-1156
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24121490
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000328941200017&DestApp=WOS_CPL

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