論文

査読有り
2012年6月

Prostacyclin receptor in tumor endothelial cells promotes angiogenesis in an autocrine manner

CANCER SCIENCE
  • Takahiro Osawa
  • ,
  • Noritaka Ohga
  • ,
  • Yasuhiro Hida
  • ,
  • Kazuko Kitayama
  • ,
  • Kosuke Akiyama
  • ,
  • Yuichiro Onodera
  • ,
  • Manabu Fujie
  • ,
  • Nobuo Shinohara
  • ,
  • Masanobu Shindoh
  • ,
  • Katsuya Nonomura
  • ,
  • Kyoko Hida

103
6
開始ページ
1038
終了ページ
1044
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1349-7006.2012.02261.x
出版者・発行元
WILEY-BLACKWELL

Molecules highly expressed in tumor endothelial cells (TEC) are important for specific targeting of these cells. Previously, using DNA microarray analysis, we found that the prostacyclin receptor (IP receptor) gene was upregulated in TEC compared with normal endothelial cells (NEC). Although prostacyclin is implicated in re-endothelialization and angiogenesis, its role remains largely unknown in TEC. Moreover, the effect of the IP receptor on TEC has not been reported. In the present study we investigated the function of the IP receptor in TEC. The TEC were isolated from two types of human tumor xenografts in nude mice, while NEC were isolated from normal counterparts. Prostacyclin secretion levels in TEC were significantly higher than those in NEC, as shown using ELISA. Real-time RT-PCR showed that the IP receptor was upregulated in TEC compared with NEC. Furthermore, migration and tube formation of TEC were suppressed by the IP receptor antagonist RO1138452. Immunohistostaining showed that the IP receptor was specifically expressed in blood vessels of renal cell carcinoma specimens, but not in glomerular vessels of normal renal tissue. These findings suggest that the IP receptor is a TEC-specific marker and might be a useful therapeutic target. (Cancer Sci 2012; 103: 10381044)

Web of Science ® 被引用回数 : 20

リンク情報
DOI
https://doi.org/10.1111/j.1349-7006.2012.02261.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22380928
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000304758200009&DestApp=WOS_CPL

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