論文

査読有り
2016年11月

The role of serotonergic mechanism in the rat prefrontal cortex for controlling the micturition reflex: An in vivo microdialysis study

NEUROUROLOGY AND URODYNAMICS
  • Hiroki Chiba
  • ,
  • Takahiko Mitsui
  • ,
  • Takeya Kitta
  • ,
  • Yu Ohmura
  • ,
  • Kimihiko Moriya
  • ,
  • Yukiko Kanno
  • ,
  • Mitsuhiro Yoshioka
  • ,
  • Nobuo Shinohara

35
8
開始ページ
902
終了ページ
907
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/nau.22843
出版者・発行元
WILEY-BLACKWELL

AimsTo investigate the role of the PFC in the micturition reflex using an in vivo microdialysis study in rats.
MethodsAdult female Sprague-Dawley rats were used and microdyalysis in the PFC and cystometrography (CMG) were performed under consciousness and free movement in the present study. Experiment 1: Samples including extracellular neurotransmitters were collected by microdyalysis and analyzed by high performance liquid chromatography. At the same time, CMG were performed to measure intercontraction interval (ICI) and maximum voiding pressure (MVP). Experiment 2: SSRI (citalopram, 1 mu M) was administered into the PFC, and microdyalysis and cystometrography (CMG) were performed simultaneously. Experiment 3: Following SSRI administration, 5-HT1A agonist (8-OH-DPAT, 300 mu M), which has the effect of decreasing the level of serotonin (5-HT) in the PFC, was administered into the PFC, and microdyalysis and CMG were performed simultaneously.
ResultsExperiment 1: Extracellular level of 5-HT in the PFC significantly increased during micturition reflex (P<0.05), whereas levels of glutamate or dopamine were not significantly changed. Experiment 2: Local administration of SSRI in the PFC increased the 5-HT level up to approximate 600% of the basal level. It also significantly increased ICI (P<0.05), whereas no significant change was found in MVP. Experiment 3: The extracellular level of 5-HT gradually decreased after local administration of 5-HT1A agonist, thereby ICI significantly decreased (P<0.05).
ConclusionsThe results of the present study suggest that the PFC has a suppressive effect on neural control of the micturition reflex via serotonin. Neurourol. Urodynam. 35:902-907, 2016. (c) 2015 Wiley Periodicals, Inc.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1002/nau.22843
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26227539
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000385842000007&DestApp=WOS_CPL

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