論文

査読有り
2015年8月

Clinical significance and therapeutic potential of prostate cancer antigen-1/ALKBH3 in human renal cell carcinoma

ONCOLOGY REPORTS
  • Kiyohiko Hotta
  • ,
  • Masayuki Sho
  • ,
  • Kiyohide Fujimoto
  • ,
  • Keiji Shimada
  • ,
  • Ichiro Yamato
  • ,
  • Satoshi Anai
  • ,
  • Hiroshi Harada
  • ,
  • Kazutake Tsujikawa
  • ,
  • Noboru Konishi
  • ,
  • Nobuo Shinohara
  • ,
  • Yoshiyuki Nakajima

34
2
開始ページ
648
終了ページ
654
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3892/or.2015.4017
出版者・発行元
SPANDIDOS PUBL LTD

Prostate cancer antigen-1 (PCA-1)/ALKBH3 has been recently identified in human prostate cancer and its expression is correlated with disease progression and prognosis. However, the precise role and function of PCA-1/ALKBH3 in human malignancies are largely unknown. In the present study, we investigated the clinical significance and therapeutic potential of PCA-1/ALKBH3 in renal cell carcinoma (RCC). PCA-1/ALKBH3 expression was examined by immunohistochemistry in 101 RCC patients who underwent radical or partial nephrectomy. Its expression was positively correlated with advanced pathological T- and M-factors and TNM stage (T, P<0.05; M, P<0.01; TNM, P<0.01, respectively). In the prognostic analysis, PCA-1/ALKBH3-negative patients with RCC had a significantly better prognosis than PCA-1/ALKBH3-positive patients (5-year survival rate, 92.9 vs. 75.9%, respectively; P<0.05). Next, the therapeutic potential of targeting PCA-1/ALKBH3 was further evaluated by small interfering RNA method using a human RCC cell line (CAKI-1). We found that PCA-1/ALKBH3 knockdown significantly inhibited the growth of CAKI-1 cells compared with the control (P<0.001). Furthermore, knockdown of PCA-1 induced apoptosis in CAKI-1 cells, as assessed by poly(ADP-ribose) polymerase-cleavage assays. We demonstrated for the first time that PCA-1/ALKBH3 expression has a significant prognostic impact on patient prognosis in RCC. Furthermore, its knockdown has a therapeutic efficacy on RCC. Taken together, both our clinical and experimental data strongly suggest that PCA-1/ALKBH3 may be functionally important and a novel molecular target for human RCC.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.3892/or.2015.4017
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26035443
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000357965600014&DestApp=WOS_CPL

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