論文

査読有り
2015年8月

Protocol for rat single muscle fiber isolation and culture

ANALYTICAL BIOCHEMISTRY
  • Yusuke Komiya
  • ,
  • Judy E. Anderson
  • ,
  • Mariko Akahoshi
  • ,
  • Mako Nakamura
  • ,
  • Ryuichi Tatsumi
  • ,
  • Yoshihide Ikeuchi
  • ,
  • Wataru Mizunoya

482
開始ページ
22
終了ページ
24
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.ab.2015.03.034
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

To attain a superior in vitro model of mature muscle fibers, we modified the established protocol for isolating single muscle fibers from rat skeletal muscle. Muscle fiber cultures with high viability were obtained using flexor digitorum brevis muscle and lasted for at least 7 days. We compared the expression levels of adult myosin heavy chain (MyHC) isoforms in these single muscle fibers with myotubes formed from myoblasts; isolated fibers contained markedly more abundant adult MyHC isoforms than myotubes. This muscle fiber model, therefore, will be useful for studying the various functions and cellular processes of mature muscles in vitro. (C) 2015 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.ab.2015.03.034
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000356398400004&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.ab.2015.03.034
  • ISSN : 0003-2697
  • eISSN : 1096-0309
  • Web of Science ID : WOS:000356398400004

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