2015年8月
Protocol for rat single muscle fiber isolation and culture
ANALYTICAL BIOCHEMISTRY
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- 巻
- 482
- 号
- 開始ページ
- 22
- 終了ページ
- 24
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ab.2015.03.034
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
To attain a superior in vitro model of mature muscle fibers, we modified the established protocol for isolating single muscle fibers from rat skeletal muscle. Muscle fiber cultures with high viability were obtained using flexor digitorum brevis muscle and lasted for at least 7 days. We compared the expression levels of adult myosin heavy chain (MyHC) isoforms in these single muscle fibers with myotubes formed from myoblasts; isolated fibers contained markedly more abundant adult MyHC isoforms than myotubes. This muscle fiber model, therefore, will be useful for studying the various functions and cellular processes of mature muscles in vitro. (C) 2015 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.ab.2015.03.034
- ISSN : 0003-2697
- eISSN : 1096-0309
- Web of Science ID : WOS:000356398400004