1997年6月
Practical scan speed in atomic force microscopy for live neurons in a physiological solution
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS SHORT NOTES & REVIEW PAPERS
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- 巻
- 36
- 号
- 6B
- 開始ページ
- 3877
- 終了ページ
- 3880
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1143/JJAP.36.3877
- 出版者・発行元
- JAPAN J APPLIED PHYSICS
We examined the practical scan speed for the observation of live neurons in a physiological solution using atomic force microscopy (AFM) with a desired vertical resolution of the order of 10(-8) mi which was reasonable when taking into account that a flicker of extracellular protein and saccharide on the neurons in the solution occurred during an observation period of a couple of minutes. The practical scan speed was found to be under 40 mu m/s, therefore, if we applied AFM using 100 lines and 100 pixels per line to an observation area of 20 mu m x 20 mu m, the minimum period for acquiring one image was estimated to be about 2 min. This procedure gave us good images that represented the slow three-dimensional dynamics in live neurons, such as the retrograde movement of surface protuberances, but suggested that another approach was required to detect fast structural changes induced by stimulation.
- リンク情報
- ID情報
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- DOI : 10.1143/JJAP.36.3877
- ISSN : 0021-4922
- CiNii Articles ID : 30021828593
- Web of Science ID : WOS:000073628300021