We cloned cDNA encording vanilloid receptor (TRPV1) from porcine and human spinal cord cDNA library. We established in vitro assay system for monitoring vanilloid receptor activities. Heterologous expressed TRPV1 receptors are activated by not onl...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(特定領域研究(B), 特定領域研究)
Project Year: 2001 - 2005 Investigator(s): Shunji KAYA
(1) Protomeric images of Na/K-ATPase and H/K-ATPase were observed by electron microsoopy(EM) after rotary-shadowing the solubilized enzyme preparation. Without cross-linking the preparation, dimeric and tetrameric molecules were detected by EM, in...
Reversible phosphorylations of Tyr^7 and Tyr^<10> of pig stomach H/K-ATPase α-chain were initially demonstrated in vivo in rat, rabbit and. These data and others also suggest that some important enzyme systems are present in the apical membrane an...
Since the discovery of Na/K-ATPase, evidence has accumulated to suggest that 1 mol of ATP hydrolysis occurs via the Na^+-occluded ADP-sensitive phosphoenzyme, the K^+- sensitive phoshphoenzyme and the K^+-occluded enzyme accompanying active transp...
