2009年1月
Trans-Translation is Involved in the CcpA-Dependent Tagging and Degradation of TreP in Bacillus subtilis
JOURNAL OF BIOCHEMISTRY
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- 巻
- 145
- 号
- 1
- 開始ページ
- 59
- 終了ページ
- 66
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1093/jb/mvn143
- 出版者・発行元
- OXFORD UNIV PRESS
TreP [trehalose-permease (phosphotransferase system (PTS) trehalose-specific enzyme IIBC component)] is one of the target proteins of tmRNA-mediated trans-translation in Bacillus subtilis [Fujihara et al. (2002) Detection of tmRNA-mediated trans-translation products in Bacillus subtilis. Genes Cells, 7, 343350]. The TreP synthesis is subject to CcpA-dependent carbon catabolite repression (CCR), and the treP gene contains catabolite-responsive element (cre) sequence, a binding site of repressor protein CcpA, in the coding region. Here, we demonstrated that the tmRNA-tagging of TreP occurs depending on the gene for CcpA. In the presence of CcpA, the transcription of treP mRNA terminates at 89 nucleotides upstream of the 5-edge of the internal cre sequence, and translational switch to the tag-sequence occurs at the 101st amino-acid (asparagine) position from N-terminus of TreP. The results show that trans-translation reaction is involved in the tagging and degradation of the N-terminal TreP fragment produced by truncated mRNA, which is a product of transcriptional roadblock by CcpA binding to the cre sequence in the internal coding region.
- リンク情報
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- DOI
- https://doi.org/10.1093/jb/mvn143
- CiNii Articles
- http://ci.nii.ac.jp/naid/10024914415
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/18977770
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000261997400008&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1093/jb/mvn143
- ISSN : 0021-924X
- CiNii Articles ID : 10024914415
- PubMed ID : 18977770
- Web of Science ID : WOS:000261997400008