論文

査読有り
2016年9月

Distinct isoform-specific complexes of TANGO1 cooperatively facilitate collagen secretion from the endoplasmic reticulum

MOLECULAR BIOLOGY OF THE CELL
  • Miharu Maeda
  • ,
  • Kota Saito
  • ,
  • Toshiaki Katada

27
17
開始ページ
2688
終了ページ
2696
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1091/mbc.E16-03-0196
出版者・発行元
AMER SOC CELL BIOLOGY

Collagens synthesized within the endoplasmic reticulum (ER) are too large to fit in conventional COPII-coated transport vesicles; thus their export from the ER requires specialized factors. TANGO1 (L) is an integral membrane protein that binds to collagen and the coatomer of vesicles and is necessary for collagen secretion from the ER. Here we characterized the short isoform of TANGO1 (TANGO1S), lacking the collagen-binding domain, and found that it was independently required for collagen export from the ER. Moreover, we found that each of the TANGO1 isoforms forms a stable protein complex with factors involved in collagen secretion: TANGO1L/cTAGE5/Sec12 (900 kDa) and TANGO1S/cTAGE5/Sec12 (700 kDa). Of interest, TANGO1S and TANGO1L seemed to be interchangeable in exporting collagen from the ER. Our results suggest that mammalian ER exit sites possess two different-sized membrane-bound macromolecular complexes that specifically function in large-cargo export from the ER.

リンク情報
DOI
https://doi.org/10.1091/mbc.E16-03-0196
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27413011
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000382889600006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1091/mbc.E16-03-0196
  • ISSN : 1059-1524
  • eISSN : 1939-4586
  • PubMed ID : 27413011
  • Web of Science ID : WOS:000382889600006

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