2017年5月
Molecular Epidemiology of Rotavirus Gastroenteritis in Central Kenya Before Vaccine Introduction, 2009-2014
JOURNAL OF MEDICAL VIROLOGY
- 巻
- 89
- 号
- 5
- 開始ページ
- 809
- 終了ページ
- 817
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/jmv.24691
- 出版者・発行元
- WILEY
Between July 2009 and June 2014, a total of 1,546 fecal specimens were collected from children <5 years of age with acute gastroenteritis admitted to Kiambu County Hospital, Central Kenya. The specimens were screened for group A rotavirus (RVA) using ELISA, and RVA-positive specimens were subjected to semi-nested RT-PCR to determine the G and P genotypes. RVA was detected in 429/1,546 (27.5%) fecal specimens. RVA infections occurred in all age groups <59 months, with an early peak at 6-17 months. The infections persisted year-round with distinct seasonal peaks depending on the year. G1P[8] (28%) was the most predominant genotype, followed by G9P[8] (12%), G8P[4] (7%), G1P[4] (5%), G9P[4] (4%), and G12P[6] (3%). In the yearly change of G and P genotypes, a major shift from G9P[8] to G1P[8] was found in 2012. Phylogenetic analysis of the nucleotide sequences of the VP7 and VP4 genes of seven strains with unusual G8 or P[6] showed that the VP7 nucleotide sequences of G8 were clustered in lineage 6 in which African strains are included, and that there are at least two distinct VP4 nucleotide sequences of P[6] strains. These results represent basic data on RVA strains circulating in this region before vaccine introduction. (C) 2016 Wiley Periodicals, Inc.
- リンク情報
- ID情報
-
- DOI : 10.1002/jmv.24691
- ISSN : 0146-6615
- eISSN : 1096-9071
- PubMed ID : 27648929
- Web of Science ID : WOS:000397953100008