J-GLOBAL         Last updated: Nov 12, 2019 at 16:47

Research Areas


Academic & Professional Experience

Professor, Kansai University Faculty of Chemistry, Materials and Bioengineering Department of Life Science and Biotechnology
Osaka University/Assistant Professor
Osaka University/Associate Professor
Kansai University/Associate Professor
- Kansai Univesity/Professor


Fermentation Technology, Graduate School, Division of Engineering, Osaka University
Fermentation Technology, Faculty of Engineering, Osaka University

Committee Memberships

- Other

Awards & Honors

Saitoh Prize
The Society Prize
The Society Prize

Published Papers

土戸 哲明
日本防菌防黴学会誌 = Journal of Antibacterial and Antifungal Agents   47(5) 217-221   May 2019
土戸 哲明
日本食品保蔵科学会誌 = Food preservation science   45(3) 149-152   May 2019
Shu Ishikawa, Shohei Ueno, Mai Mitsui, Yoshinobu Matsumura, Tetsuro Hatsuoka
Biocontrol Science   24 57-65   Jan 2019
© 2019 Arab Society for Plant Protection. All rights reserved. Effective spatial disinfection systems are required for human health care, public hygiene, and food and medicine manufacturing. Although some aerosolized disinfectants were already app...
Sakai Toshio, Tsuchido Tetsuaki, Furuta Masakazu
BIOCONTROL SCIENCE   23(3) 121-128   Sep 2018   [Refereed]
Tsuchido Tetsuaki, Sakamoto Jin
Tsuchido Tetsuaki
Tsuchido Tetsuaki
BIOCONTROL SCIENCE   22(2) 131-135   Jun 2017   [Refereed]
土戸 哲明
イルシー   (130) 11-17   May 2017
Shimazaki Aiko, Sakamoto Jin J., Furuta Masakazu, Tsuchido Tetsuaki
BIOCONTROL SCIENCE   21(2) 123-130   Jun 2016   [Refereed]
原田 真美, 坂元 仁, 古田 雅一, 高鳥 浩介, 土戸 哲明
The journal of Center for the Global Study of Cultural Heritage and Culture   4 85-99   2016
高橋 淳子, 太田 利子, 久米田 裕子, 高鳥 美奈子, 土戸 哲明, 高鳥 浩介
The journal of Center for the Global Study of Cultural Heritage and Culture   4 61-73   2016
土戸 哲明, 坂元 仁
Foods & food ingredients journal of Japan : FFIジャーナル   221(4) 278-283   2016
Ihara Nozomi, Sakamoto Jin, Yoshida Munehiro, Tsuchido Tetsuaki
BIOCONTROL SCIENCE   20(2) 91-97   Jun 2015   [Refereed]
高橋 淳子, 村松 芳多子, 太田 利子, 土戸 哲明, 高鳥 浩介
The journal of Center for the Global Study of Cultural Heritage and Culture   3 83-98   2015
原田 真美, 坂元 仁, 古田 雅一, 高鳥 浩介, 土戸 哲明
The journal of Center for the Global Study of Cultural Heritage and Culture   3 73-81   2015
Heat Sterilization and Pasteurization
Tsuchido Tetsuaki
FOOD HYGIENE AND SAFETY SCIENCE   55(6) J211-J214   Dec 2014   [Refereed]
井原 望, 濱田 信夫, 土戸 哲明
日本防菌防黴学会誌 = Journal of Antibacterial and Antifungal Agents   42(2) 63-71   Feb 2014
土戸 哲明, 坂元 仁
日本食品保蔵科学会誌 = Food preservation science   39(2) 109-113   Mar 2013
Tomoko Abe, Jin Sakamoto, Tetsuaki Tsuchido, Ichiro Nakamura
japan journal of food engineering   13 137-142   Jan 2012
We made a re-expanded version for the database of thermal death of microorganisms, ThermoKill Database (TKDB) R8110. The database was built using data adopted and collected from research papers published between 1981 and 2010 in 25 major selected ...
防菌防黴 = Journal of antibacterial and antifungal agents   39, (12), 759-766(12) 759-766   Dec 2011
All genomic mutations in the antimicrobial surfactant-resistant mutant, Escherichia coli OW66, are involved in cell resistance to surfactant.
Nakata K, Koh MM, Tsuchido T, Matsumura Y
87(5) 1895-1905   Aug 2010   [Refereed]
Shigemoto Mayumi, Nakagawa Kayo, Sakamoto Jin J., Tsuchido Tetsuaki
BIOCONTROL SCIENCE   15(1) 27-31   Mar 2010   [Refereed]
Matsumura Y, Yamada K, Takahashi M, Kikuchi Y, Tsuchido T
Biocontrol science   12(1) 21-29   Mar 2007   [Refereed]
Abe T, Nakamura I, Yokohara T, Omura Y, Hiraga T, Tsuchido T
Biocontrol science   12(1) 35-38   Mar 2007   [Refereed]
Sasaki M, Akahira A, Oshiman K, Tsuchido T, Matsumura Y
Applied and environmental microbiology   71(12) 8024-8030   Dec 2005   [Refereed]
Sasaki M, Maki J, Oshiman K, Matsumura Y, Tsuchido T
Biodegradation   16(5) 449-459   Oct 2005   [Refereed]
Tetsuaki Tsuchido
Biocontrol Science   10 1   Jun 2005
A. Jagannath, T. Tsuchido, J. M. Membré
Food Microbiology   22 233-239   Apr 2005
The association of a modified Weibull model and Bigelow model was applied to the thermal inactivation of Bacillus subtilis spores heated in phosphate buffer, milk, kayu (a Japanese style rice porridge) and soy sauce as well. The inactivation kinet...
A. Jagannath, T. Tsuchido
Letters in Applied Microbiology   37 399-404   Nov 2003
Aims: The predicted survival of Bacillus subtilis 168 spores from a polynomial regression equation was validated in milk. Methods and Results: Bias factor suggested as an index of model performance was used to validate the polynomial model predict...
A. Jagannath, I. Nakamura, T. Tsuchido
Journal of Applied Microbiology   95 135-141   Jul 2003
Aims: The inactivation of Bacillus subtilis 168 spores subjected to the combined stress of pH, temperature and sodium chloride in a buffer system was modelled. Methods and Results: Bacillus subtilis 168 spore suspension in 50 mmol l-1 potassium ph...
Matsumura Y, Yoshikata K, Kunisaki S, Tsuchido T
Applied and environmental microbiology   69(7) 4278-4281   Jul 2003   [Refereed]
A. Jagannath, Tetsuaki Tsuchido
Biocontrol Science   8 1-7   Jan 2003
Yoshihiko Omura, Mayumi Shigemoto, Takahiro Akiyama, Hiroyuki Saimoto, Yoshihiro Shigemasa, Ichiro Nakamura, Tetsuaki Tsuchido
Biocontrol Science   8 25-30   Jan 2003
The antimicrobial activity of chitosan with different molecular weights (MWs) and different degrees of acetylation (DA) was examined to clarify how the activity depends upon the molecular structures. We determined minimum growth inhibitory concent...
Preparations of heterospirostanols and their pharmacological activities.
Quan HJ, Koyanagi J, Ohmori K, Uesato S, Tsuchido T, Saito S
European journal of medicinal chemistry   37(8) 659-669   Aug 2002   [Refereed]
Kitagawa M, Miyakawa M, Matsumura Y, Tsuchido T
European journal of biochemistry   269(12) 2907-2917   Jun 2002   [Refereed]
Jin J. Sakamoto, Keiko Minami, Tetsuaki Tsuchido
Biocontrol Science   7 63-74   Jan 2002
When the log-phase cells of Bacillus subtilis 168 were exposed to a cold shock drop in temperature from 37 to 0 °C followed by incubation at 37 °C or a heat shock rise in temperature from 37 to 55 °C, their chromosomal DNA was found to be cleaved,...
Jin J. Sakamoto, Miho Sasaki, Tetsuaki Tsuchido
Journal of Biological Chemistry   276 47046-47051   Dec 2001
We purified and characterized a 39-kDa Bacillus subtilis 168 nuclease that has been suggested in this laboratory to be involved in chromosomal DNA degradation induced by lethal heat and cold shock treatments in vivo. The nuclease activity was inhi...
Hiroyuki Yamanaka, Tetsuaki Tsuchido
Biocontrol Science   5 17-23   Mar 2000
We investigated the death kinetics for Escherichia coli K12 cells heated at different temperatures in the presence of saturated fatty acids having short and medium alkyl-chain lengths at various concentrations. Apparent first-order death kinetics ...


藤山 貴友, 松浦 寛人, 坂元 仁, 土戸 哲明, 古田 雅一
電気学会研究会資料. PPT = The papers of technical meeting on pulsed power technology, IEE Japan   2017(18) 17-19   May 2017
藤山 貴友, 松浦 寛人, 坂元 仁, 土戸 哲明, 古田 雅一
電気学会研究会資料. ED = The papers of technical meeting on electrical discharges, IEE Japan   2017(38) 17-19   May 2017
藤山 貴友, 松浦 寛人, 坂元 仁, 土戸 哲明, 古田 雅一
電気学会研究会資料. PST   2017(18) 17-19   May 2017
土戸 哲明, 坂元 仁
食品工業   55(10) 45-53   May 2012
防菌防黴 = Journal of antibacterial and antifungal agents   40(5) 297-298   May 2012
Teramura Kenichirou, Sakamoto Jin, Keisuke Nishio, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   64    2012
Teramura Kenichiro, Sakamoto Jin, Tsuchido Tetuaki
日本生物工学会大会講演要旨集   63    2011
TUCHIDO Tetsuaki
防菌防黴 = Journal of antibacterial and antifungal agents   38(9) 587-595   Sep 2010
土戸 哲明
Food processing and ingredients   45(1) 4-6   Jan 2010
NAKATA Kunihiro, TSUCHIDO Tetsuaki, MATSUMURA Yoshinobu
日本生物工学会大会講演要旨集   22    2010
TERAMURA Kenichirou, GONJYO Tomoka, SAIGAN Fumiya, SAKAMOTO Jin, TSUCHIDO Tetsuaki
日本生物工学会大会講演要旨集   22    2010
Japanese journal of food microbiology   26(2) 70-75   Jul 2009
防菌防黴 = Journal of antibacterial and antifungal agents   37(1) 43-49   Jan 2009
Japan Journal of Food Engineering   10(4) 183-190   Dec 2009
The results obtained from our studies on several aspects of food pasteurization and sterilization by cross-talk between microbiology and technology were presented. First, we found that the thermal death of Escherichia coli was affected by r...
Sakamoto Jin, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   21    2009
Japanese Journal of Food Microbiology   26(2) 70-75   2009
MEMBRANE   33(6) 266-272   Nov 2008
The cell membrane plays a critical role for discrimination of the cellular inner world from the outside environment.When bacterial cells are exposed to a certain environmental stress, their plasma membrane may be the first sensorespecially for its...
土戸 哲明, 坂元 仁
The Food industry   51(14) 20-26   Jul 2008
防菌防黴 = Journal of antibacterial and antifungal agents   36(6) 403-409   Jun 2008
Tetsuaki Tsuchido
Technology Reports of Kansai University   50    Mar 2008
Tadao Oikawa, Shinichi Uesato, Hiroshi Tamura, Hidehisa Kawahara, Yasuo Nagaoka, Koji Shimoke, Tetsuaki Tsuchido, Lothar Eggeling, Volker F. Wendisch, Jochen Büchs, Ratana Rujiravanit, Nazalan Najimudin, Chan Lai Keng
Technology Reports of Kansai University   50 83-94   Mar 2008
We proposed an "International Network Exchange Program of Education and Research for Graduate Course Students in Life Science and Biotechnology" to the Kansai University Special Research Fund 2007 in order to promote international student exchange...
日本生物工学会大会講演要旨集   20    2008
Buchs Jochen, Eggeling Lothar, Wendisch Volker F., Chan Lai Keng, Mohd Nazalan bin, Mohd Najimudin, Rujiravanit Ratana
Engineering & technology   15 81-89   2008
防菌防黴 = Journal of antibacterial and antifungal agents   35(1) 1-2   Jan 2007
TSUCHIDA Shohei, MAEKAWA Chikano, OOSHIMAN Ko-ichi, TSUCHIDO Tetsuaki, MATSUMURA Yoshinobu
日本生物工学会大会講演要旨集   19    2007
防菌防黴 = Journal of antibacterial and antifungal agents   34(8) 497-506   Aug 2006
Japanese Journal of Food Microbiology   23(2) 41-52   Jul 2006
TAMURA Hiroshi, SANO Hirofumi, NEGORO Isao, TOKURA Seiichi, TSUCHIDO Tetsuaki
キチン・キトサン研究 = Chitin and chitosan research   12(2) 198-199   Jul 2006
Yoshinobu Matsumura, Shuhei Nishikawa, Hiroki Tanaka, Tetsuaki Tsuchido
Technology Reports of Kansai University   48 63-70   Mar 2006
It is known that peracetic acid (PAA) is one of the few powerful antimicrobial agents available for use as a sporicidal agent. In this study, the antimicrobial action of a commercial preparation of PAA is characterized and the effects of chemicals...
Cryobiology and Cryotechnology   52(1) 35-42   2006
Food-borne pathogenic and spoilage bacteria can be killed by various methods in sterilization and pasteurization processes. In the process for minimizing the reduction in food quality, after the processing, bacterial cells often do not die but are...
YAMAZAKI Syunsuke, IWASAKI Shinya, OKANO Tetsuya, TSUTIDO Tetsuaki
日本生物工学会大会講演要旨集   18    2006
日本生物工学会大会講演要旨集   17    2005
防菌防黴 = Journal of antibacterial and antifungal agents   32(5) 251-263   May 2004
Japanese Journal of Food Microbiology   20(4) 141-149   Dec 2003
TOKUDA Satoshi, YAMAMOTO Yasuhumi, UEMURA Tomomasa, MATSUMURA Yoshinobu, TSUCHIDO Testuaki
Journal of the Visualization Society of Japan   23(1) 451-452   Jul 2003
In the field of biotechnology at present, in order to evaluate the viability of bacteria, colony counting is generally used. However, colony counting requires 10 to 48 hours, so delicate changes with passage of time cannot be measured. In this stu...
Japan Journal of Food Engineering   3(2) 41-45   Jan 2002
For the construction of functional predictive models describing thermal death behaviors of foodborne spoilage and pathogenic microorganisms in foods, abundant thermal death data should be collected. We have been carrying out the build-up of therma...
Hayashi Kinumi, Sakamoto Jin, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   15    2003
Sasaki Miho, Matsumura Yoshinobu, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   15    2003
Iwasaki Shinya, Sasaki Miho, Matsumura Yoshinobu, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   15    2003
Tsuchido Tetsuaki
Engineering & technology   12(4) 33-36   Nov 2002
Journal of antibacterial and antifungal agents,Japan   30(2) 105-110   Feb 2002
Tsuchido Tetsuaki
関西大学工業技術研究所研究報告   17 55-65   2002
Tsuchido Tetsuaki
関西大学工業技術研究所研究報告   17 72-78   2002
Sakamoto Jin, Kato Hiromichi, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   14    2002
Sasaki Miho, Iwasaki shinya, Matsumura Yoshinobu, Tuchido Tetsuaki
日本生物工学会大会講演要旨集   14    2002
Hosokawa Chiemi, Iwasaki Shinya, Sasaki Miho, Matsumura Yoshinobu, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   14    2002
Hayashi Kinumi, Nakamoto Hideomi, Sakamoto Jin, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   14    2002
Miyawaki Shunsuke, Yoshida Yuri, Kagimoto Yumiko, Matumura Yoshinobu, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   14    2002
Abe Takanori, Kamei Seiichiro, Ikeda Takumi, Matsumura Yoshinobu, Tsuchido Tetsuaki
日本生物工学会大会講演要旨集   14    2002
Hyomen Kagaku   22(10) 645-651   Oct 2001
The cell surface changes sensitively with environmental stimuli because it locates at the interface between the cell and the environment. Bacteria have their elaborate systems against a variety of stresses as well as other organisms and possess ab...
SHIGEMOTO Mayumi, OMURA Yoshihiko, NAKAMURA Ichiro, TSUCHIDO Tetsuaki
キチン・キトサン研究 = Chitin and chitosan research   7(2) 120-121   Jul 2001
TSUCHIDO Tetsuaki, HAYASHI Masahi, MATSUMURA Yoshinobu, OHYA Seiji
Journal of antibacterial and antifungal agents,Japan   29(5) 305-309   May 2001
MATSUMURA Yoshinobu, TSUCHIDO Tetsuaki
Journal of High Temperature Society.   27(1) 35-41   Jan 2001
Journal of antibacterial and antifungal agents,Japan   28(10) 623-633   Oct 2000
防菌防黴 = Journal of antibacterial and antifungal agents   28(10)    Oct 2000
Journal of antibacterial and antifungal agents,Japan   28(10) 657-662   Oct 2000
Ichiro Nakamura, Takashi Yokohara, Tetsuaki Tsuchido
Biocontrol Science   5 61-64   Mar 2000
To build a database on the thermal death of microorganisms, we designed a formulated card system consisting of more than 60 items. Those items are displayed on a card on the monitor screen of a personal computer and most of the corresponding param...
Kenji Yasunaga, Kenji Sumino, Yoshinobu Matsumura, Tetsuaki Tsuchido
Technology Reports of Kansai University   41 161-168   Dec 1999
A novel method using computer-assisted image analysis of bacterial motion was developed for evaluating water pollution. The motility of a tumbling-defective mutant of Escherichia coli, OW23 strain isolated in this study, was reduced in the presenc...
Nippon Shokuhin Kagaku Kogaku Kaishi   46(1) 1-8   Jan 1999
Tetsuaki Tsuchido
Nippon Shokuhin Kagaku Kogaku Kaishi   46 1-8   Jan 1999
Journal of antibacterial and antifungal agents,Japan   26(10) 565-569   Oct 1998
Yachio Tsuchida, Tetsuaki Tsuchido
Biocontrol Science   2 19-26   Jan 1997
We developed Mn-supplemented, Fe-supplemented, and Ca-supplemented spores of Bacillus subtilis 168 and ATCC9372 in the respective presence of manganese, ferric and calcium ions, and compared their resistance to vapor-phase hydrogen peroxide with t...
Tetsuaki Tsuchido, Kayoko Miyake, Makoto Hayashi, Katsuhiro Tamura
Progress in Biotechnology   13 185-188   Dec 1996
We confirmed our previous finding that the thermal death of Escherichia coli was affected by the holding temperature prior to heat treatment as well as growth temperature. The cell death caused by hydrostatic pressure at 150 MPa was also found to ...
TSUCHIDO Tetsuaki, MATSUMURA Yoshinobu, OKU Kiyoharu
バイオサイエンスとインダストリー = Bioscience & industry   54(4) 39-40   Apr 1996
食品衛生学雑誌   36(4) 547-549   Aug 1995
OBATA Hitoshi, KAWAHARA Hidehisa, TOKUYAMA Tai, IKUGAWA Hirokazu, TSUCHIDO Tetsuaki
防菌防黴 = Journal of antibacterial and antifungal agents   23(4) 221-226   Apr 1995
Tetsuaki Tsuchido, Yoshiaki Kato, Tomohiko Nishino, Mitsuo Takano
Bioscience, Biotechnology, and Biochemistry   59 1636-1640   Jan 1995
Exponentially growing Bacillus subtilis cells autolysed when exposed to cold shock treatment in minimal medium followed by incubation at 37°C. From characteristics of the lysis, it was suggested that the cold-shock-induced cell lysis resulted from...
H. Obata, H. Kawahara, T. Tokuyama, H. Ikugawa, T. Tsuchido
Journal of Antibacterial and Antifungal Agents, Japan   23 221-226   Jan 1995
Conditions of inducing cell lysis of ice-nucleating Xanthomonas campestris pv. translucens (XCT-1) were investigated, some being described here for the first time. Cells of strain XCT-1 were lysed after being grown on a basal medium with sodium ci...
Tetsuaki Tsuchido
Applied Microbiology and Biotechnology   41 106-109   Mar 1994
Lysophosphatidylcholine (LPC) was found to cause autolysis of Bacillus subtilis 168 cells growing logarithmically at concentrations higher than 20 μm, by inducing the activity of autolytic enzymes. The lytic activity depended upon the carbon-chain...
Tetsuaki Tsuchido, Hironori Takeuchi, Hidehisa Kawahara, Hitoshi Obata
Journal of Fermentation and Bioengineering   78 185-187   Jan 1994
The 'relative' cell injury, or conversely the degree of intactness, in a cell population subjected to stress was evaluated using image analysis of the motility of free-swimming cells of Bacillus subtilis, and compared with the cell motion in a non...
N. Eshraghi, R. H. Wainberg, T. L. Walden, T. Tsuchido, M. B. Yatvin
International Journal of Hyperthermia   10 79-88   Jan 1994
Hyperthermic treatment reduces protein synthesis and modifies amino acid transport in Escherichia coli. The present study examined the role of nutrient availability on these processes. Cultures of E. coli in log phase were aliquoted into growth me...
Koji Yano, Tetsuhiro Hasegawa, R. Ryoichi, K. Kuboi, Isao Komasawa, Tetsuaki Tsuchido
The surface properties of GroEL and GroES, typical heat shock proteins (HSPs) from E. coli, were characterized in terms of isoelectric point, surface net hydrophobicity and local hydrophobicity using aqueous two-phase systems (ATPS). The surface n...
Toshio Miyake, Shigeki Araki, Tetsuaki Tsuchido
Bioscience, Biotechnology and Biochemistry   57 578-583   Jan 1993
After heat treatment at 55°C for 15s, Escherichia coli cells were incubated at 37°C in a chemically defined medium for cell recovery from heat injury. During this period, four heat-inducible proteins with similar molecular masses of 15 kDa or so b...
Hisakazu Tanaka, Ryoichi Kuboi, Isao Komasawa, Tetsuaki Tsuchido
Journal of Fermentation and Bioengineering   75 424-429   Jan 1993
A method for the release of intracellular enzyme by autolysis of Bacillus subtilis cells is presented. Both the growth and lysis processes were further applied to aqueous two-phase systems (ATPS). Lysis induced by the addition of Triton X-100 and ...
Tetsuaki Tsuchido, Naomi Yokosuka, Mitsuo Takano
Journal of Fermentation and Bioengineering   75 191-195   Jan 1993
A spontaneous mutant of Bacillus subtilis 168, SR3, tolerant to the autolysis-inducing action of sucrose monoesters of long-chain fatty acids, was isolated. It was shown that its susceptibility to the lytic action of sucrose esters and glycerol es...
Japanese Journal of Freezing and Drying   39(0) 61-67   1993
Exposure of E. coli cells to high temperature at 50 to 55℃ results in sublethal or lethal stress on cells. Bacterial membranes have a role in the cellular resistance to heat, as suggested by the effect of membrane fluidity on the heat resistance. ...
T. Tsuchido, A. Takeuchi, M. Takano
Journal of Applied Bacteriology   73 531-534   Jan 1992
Escherichia coli cells exposed to a sublethal heat treatment at 55°C for 15s synthesized lipopolysaccharide during their recovery period after heat stress. As chloramphenicol at least partly inhibited the synthesis of lipopolysaccharide, it is sug...
T. Tsuchido, S. Naruki, I. Shibasaki
Journal of Antibacterial and Antifungal Agents, Japan   20 197-203   Jan 1992
Quenan Lin, Tetsuaki Tsuchido, Mitsuo Takano
Applied Microbiology and Biotechnology   35 585-590   Aug 1991
The photodynamic inactivation by near UV and α-terthienyl (αT) of bacteria immobilized in poly(methyl methacrylate) (PMMA) was observed. Killing curves for Escherichia coli strains exhibited multi-hit kinetics, whereas curves for Pseudomonas aerug...
Ancharida Svarachorn, Tetsuaki Tsuchido, Atsuhiko Shinmyo, Mitsuo Takano
Journal of Fermentation and Bioengineering   71 281-283   Jan 1991
By exposure to a temperature below the membrane phase transition point, Bacillus subtilis 168 lost their permeability control followed by the leakage of intracellular K+ and incapability of glucose uptake, resulting in cellular lysis in the follow...
Heon Young Cho, Tetsuaki Tsuchido, Hisayo Ono, Mitsuo Takano
Journal of Fermentation and Bioengineering   70 11-14   Jan 1990
A cationic surfactant, palmityltrimethylammonium iodide at 6 to 40 μM caused the death and lysis of Bacillus subtilis 168 cells growing logarithmically in a chemically defined medium. The relationship between the induced death and lysis was invest...
T. Tsuchido, A. Svarachorn, H. Soga, M. Takano
Antimicrobial Agents and Chemotherapy   34 781-785   Jan 1990
The surfactants tested in this study lysed Bacillus subtilis 168 cells at the logarithmic growth phase. Results obtained with inhibitors and a mutant that had defective autolytic enzymes suggested that cell lysis resulted from the deregulation of ...
Ancharida Svarachorn, Atsuhiko Shinmyo, Tetsuaki Tsuchido, Mitsuo Takano
Applied Microbiology and Biotechnology   30 299-304   Mar 1989
Monovalent cations including K+, Na+, NH4+, Cs+, Rb+, or Li+ at 100 mM concentration caused cellular lysis of Bacillus subtilis 168 in the log phase. The lysis caused by 100 mM K+ was inhibited by divalent cations at 100 mM including Mg++, Ca++, M...
T. Tsuchido, I. Aoki, M. Takano
Journal of general microbiology   135 1941-1947   Jan 1989
The fluorescent dye 1-N-phenylnaphthylamine permeated Escherichia coli cells after exposure to a heat stress at 55 degrees C in Tris/Mg2+ buffer, pH 8.0. The rate of dye permeation increased with time during heat treatment and decreased gradually ...
Tetsuaki Tsuchido, Toshihiko Koike, Mitsuo Takano
Journal of Fermentation and Bioengineering   67 132-134   Jan 1989
An assay method for the evaluation of a microbial growth-inhibition caused by an inhibitory substance was proposed by modification of the original growth-delay method, which has been reported previously [J. Ferment. Technol., 60, 189, 1982]. The u...
Ancharida Svarachorn, Atsuhiko Shinmyo, Tetsuaki Tsuchido, Mitsuo Takano
Journal of Fermentation and Bioengineering   68 252-256   Jan 1989
Autolysis of Bacillus subtilis 168 cells in buffer solution was induced by high concentrations of monovalent cations, which activate N-acetylmuramoyl-l-alanine amidase, a major autolysin in this bacterium. However, cells did not lyse in Spizizen s...
T. Tsuchido, M. Takano
Antimicrobial Agents and Chemotherapy   32 1680-1683   Jan 1988
The sensitivities of intact and heat-injured cells of Escherichia coli K-12 to several antibacterial compounds were measured by the prolongation of growth delay. Cells exposed to sublethal heat became more sensitive to various hydrophobic compound...
A. Svara-Chorn, A. Shinmyo, T. Tsuchido, M. Takano
Nippon Nogeikagaku Kaishi   62 270-279   Jan 1988
T. Tsuchido, Y. H. Ahn, M. Takano
Applied and Environmental Microbiology   53 505-508   Jan 1987
T. Tsuchido, R. A. VanBogelen, F. C. Neidhardt
Proceedings of the National Academy of Sciences of the United States of America   83 6959-6963   Jan 1986
Analysis of a mutant in fam, a pleiotropic gene affecting cell division in Escherichia coli, revealed that this gene is probably identical to the heat shock regulatory gene htpR. The fam-715 mutant and different htpR mutants were found to share th...
T. Tsuchido, N. Katsui, A. Takeuchi, M. Takano, I. Shibasaki
Applied and Environmental Microbiology   50 298-303   Nov 1985
T. Tsuchido, T. Hiraoka, M. Takano, I. Shibasaki
Journal of Bacteriology   162 42-46   Jan 1985
The addition of saturated C6, C8, C10, and C12 fatty acids appeared to lyse actively growing cells of Bacillus subtilis 168, as judged by a decrease in the optical density of the culture. Of these fatty acids, dodecanoic acid was the most effectiv...
N. Katsui, T. Tsuchido, R. Hiramatsu, S. Fujikawa, M. Takano, I. Shibasaki
Journal of Bacteriology   151 1523-1531   Dec 1982
Thermal damage to the outer membrane of E. coli W3110 was studied. When E. coli cells were heated at 55°C in 50 mM Tris-hyrochloride buffer at pH 8.0, surface blebs were formed on the cell envelope, mainly at the septa of dividing cells. Membrane ...
N. Katsui, T. Tsuchido, M. Takano, I. Shibasaki
Journal of Applied Bacteriology   53 103-108   Jan 1982
The viability of heat‐stressed cells of Escherichia coli was influenced by the temperature of incubation prior and subsequent to heat treatment. The effect of pre‐incubation temperature on the viability of heated cells was almost constant regardle...
Journal of Food Safety   3 57-68   Jan 1981
A kinetic study was performed on the combined effect of monolaurin and heat on the death of Escherichia coli. The following results were obtained: (1) Monolaurin was about eleven times more active than that of sodium laurate; (2) Temperature enhan...
N. Katsui, T. Tsuchido, M. Takano, I. Shibasaki
Journal of General Microbiology   122 357-361   Jan 1981
T. Tsuchido, I. Shibasaki
Biotechnology and Bioengineering   22 107-118   Jan 1980
Studies were made of the death kinetics of Escherichia coli cells heated at 46 to 56°C in 0.05M phosphate buffer (pH 7.0) containing either an amphoteric surfactant (Tego 15DL, 1–10 μg/ml) or sorbic acid (0.5 to 3%). A linear relationship was obta...

Conference Activities & Talks


Research Grants & Projects

Cellular Injury, repair and response in heat-stressed bacteria
Project Year: 1972   
Properties and mechanisms of action of antimicrobial agents
Funded Research
Project Year: 1972   
Mechanism of Self-degradation of Bacteria
Project Year: 1982   
Mechanism of Defense to Oxidative Stress in Bacterial Cells