2005年7月
Extracellular superoxide dismutase overexpression reduces cuff-induced arterial neointimal formation
ATHEROSCLEROSIS
- 巻
- 181
- 号
- 1
- 開始ページ
- 55
- 終了ページ
- 62
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.atherosclerosis.2005.01.051
- 出版者・発行元
- ELSEVIER IRELAND LTD
The mechanisms of neointimal formation in cuff-injury models are still uncertain. To examine whether extracellular superoxide dismutase (EC-SOD) can reduce neointimal formation in a cuff-injury model, adenoviruses expressing EC-SOD (AxCAEC-SOD) or Escherichia coli beta-galactosidase (AxCALacZ) was injected between the cuff and the adventitia of rat femoral arteries. As a result, EC-SOD protein was effectively produced in the adventitia, as assessed by immunohistochemical staining. In comparison with cuff-treated control arteries and AxCALacZ-transfected arteries, neointimal formation was significantly reduced in AxCAEC-SOD-transfected arteries. Furthermore, proliferating smooth muscle cells in neointima and media were reduced by EC-SOD treatment. Similarly, augmented iNOS expression, apoptosis and collagen content in the vascular wall were also reduced by EC-SOD treatment. Reactive oxygen species (ROS) generation in tissue was reduced by EC-SOD expression, as assessed by dihydroethidium staining and coelenterazine chemiluminescence. These results suggest that ROS, especially superoxide anions at an adventitia, are responsible for neointimal formation in a cuff-injury model. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.atherosclerosis.2005.01.051
- ISSN : 0021-9150
- Web of Science ID : WOS:000230215300007