MISC

2007年6月

Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Ayako Koizumi
  • ,
  • Ken-ichiro Nakajima
  • ,
  • Tomiko Asakura
  • ,
  • Yuji Morita
  • ,
  • Keisuke Ito
  • ,
  • Akiko Shmizu-Ibuka
  • ,
  • Takumi Misaka
  • ,
  • Keiko Abe

358
2
開始ページ
585
終了ページ
589
記述言語
英語
掲載種別
DOI
10.1016/j.bbrc.2007.04.171
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

This study examines taste reception of neoculin, a Curculigo latifolia sweet protein with taste-modifying activity which converts sourness to sweetness. Neoculin tastes sweet to humans, but not to mice, and is received by the human sweet taste receptor hT1R2-hT1R3. In the present study with calcium imaging analysis of HEK cells expressing human and mouse T1Rs, we demonstrated that hT1R3 is required for the reception of neoculin. Further experiments using human/mouse chimeric T1R3s revealed that the extracellular amino terminal domain (ATD) of hT1R3 is essential for the reception of neoculin. Although T1R2-T1R3 is known to have multiple potential ligand-binding sites to receive a wide variety of sweeteners, the present study is apparently the first to identify the ATD of hT1R3 as a new sweetener-binding region. (C) 2007 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2007.04.171
CiNii Articles
http://ci.nii.ac.jp/naid/80018450430
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17499612
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000246927300033&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2007.04.171
  • ISSN : 0006-291X
  • CiNii Articles ID : 80018450430
  • PubMed ID : 17499612
  • Web of Science ID : WOS:000246927300033

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