2007年6月
Taste-modifying sweet protein, neoculin, is received at human T1R3 amino terminal domain
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- 巻
- 358
- 号
- 2
- 開始ページ
- 585
- 終了ページ
- 589
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.bbrc.2007.04.171
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
This study examines taste reception of neoculin, a Curculigo latifolia sweet protein with taste-modifying activity which converts sourness to sweetness. Neoculin tastes sweet to humans, but not to mice, and is received by the human sweet taste receptor hT1R2-hT1R3. In the present study with calcium imaging analysis of HEK cells expressing human and mouse T1Rs, we demonstrated that hT1R3 is required for the reception of neoculin. Further experiments using human/mouse chimeric T1R3s revealed that the extracellular amino terminal domain (ATD) of hT1R3 is essential for the reception of neoculin. Although T1R2-T1R3 is known to have multiple potential ligand-binding sites to receive a wide variety of sweeteners, the present study is apparently the first to identify the ATD of hT1R3 as a new sweetener-binding region. (C) 2007 Elsevier Inc. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.bbrc.2007.04.171
- CiNii Articles
- http://ci.nii.ac.jp/naid/80018450430
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/17499612
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000246927300033&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.bbrc.2007.04.171
- ISSN : 0006-291X
- CiNii Articles ID : 80018450430
- PubMed ID : 17499612
- Web of Science ID : WOS:000246927300033