2000年2月
Cell surface expression of immature H glycoprotein in measles virus-infected cells
VIRUS RESEARCH
- 巻
- 66
- 号
- 2
- 開始ページ
- 187
- 終了ページ
- 196
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/S0168-1702(00)00124-6
- 出版者・発行元
- ELSEVIER SCIENCE BV
Two forms of hemagglutinin (H) protein, one with an apparent molecular mass of 78 kDa (78K H protein) and the other with that of 74 kDa (74K H protein), are present in cells infected with measles virus (MV). We previously observed that only the mature 78K H protein, a completely glycosylated form of the 74K H protein, was expressed on the cell surface of the infected cells. In the present study, we detected transient expression of the 74K H protein on the cell surface of infected cells by pulse-chase studies, although the level of this expression was much lower than that of the 78K H protein. On the cell surface the 74K H protein was present as dimers and sensitive to endo-beta-N-acetylglucosaminidase H digestion. Treatment with brefeldin A, which blocks the transport of membrane and secretory proteins from the endoplasmic reticulum to the Golgi apparatus, inhibited the cell surface expression of the 78K H protein, but not that of the 74K H protein. These data suggest that a part of the MV 74K H proteins could be transported directly to the cell surface - probably via an alternative pathway - without processing to the complex form in the Golgi apparatus. (C) 2000 Elsevier Science B.V. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/S0168-1702(00)00124-6
- CiNii Articles
- http://ci.nii.ac.jp/naid/80011843465
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10725551
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000086183800008&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1016/S0168-1702(00)00124-6
- ISSN : 0168-1702
- CiNii Articles ID : 80011843465
- PubMed ID : 10725551
- Web of Science ID : WOS:000086183800008