MISC

2000年2月

Cell surface expression of immature H glycoprotein in measles virus-infected cells

VIRUS RESEARCH
  • H Ogura
  • Matsunaga, I
  • Y Takano
  • XJ Ning
  • M Ayata
  • K Tanaka
  • T Seto
  • K Furukawa
  • N Ito
  • M Shingai
  • T Kimura
  • K Ichihara
  • H Kubo
  • T Murakami
  • 全て表示

66
2
開始ページ
187
終了ページ
196
記述言語
英語
掲載種別
DOI
10.1016/S0168-1702(00)00124-6
出版者・発行元
ELSEVIER SCIENCE BV

Two forms of hemagglutinin (H) protein, one with an apparent molecular mass of 78 kDa (78K H protein) and the other with that of 74 kDa (74K H protein), are present in cells infected with measles virus (MV). We previously observed that only the mature 78K H protein, a completely glycosylated form of the 74K H protein, was expressed on the cell surface of the infected cells. In the present study, we detected transient expression of the 74K H protein on the cell surface of infected cells by pulse-chase studies, although the level of this expression was much lower than that of the 78K H protein. On the cell surface the 74K H protein was present as dimers and sensitive to endo-beta-N-acetylglucosaminidase H digestion. Treatment with brefeldin A, which blocks the transport of membrane and secretory proteins from the endoplasmic reticulum to the Golgi apparatus, inhibited the cell surface expression of the 78K H protein, but not that of the 74K H protein. These data suggest that a part of the MV 74K H proteins could be transported directly to the cell surface - probably via an alternative pathway - without processing to the complex form in the Golgi apparatus. (C) 2000 Elsevier Science B.V. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/S0168-1702(00)00124-6
CiNii Articles
http://ci.nii.ac.jp/naid/80011843465
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10725551
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000086183800008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/S0168-1702(00)00124-6
  • ISSN : 0168-1702
  • CiNii Articles ID : 80011843465
  • PubMed ID : 10725551
  • Web of Science ID : WOS:000086183800008

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