Aug, 2014
Biased hypermutation occurred frequently in a gene inserted into the IC323 recombinant measles virus during its persistence in the brains of nude mice
VIROLOGY
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- Volume
- 462
- Number
- First page
- 91
- Last page
- 97
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.1016/j.virol.2014.05.035
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Measles virus (MV) is the causative agent of measles and its neurological complications, subacute sclerosing panencephalitis (SSPE) and measles inclusion body encephalitis (MIBE). Biased hypermutation in the M gene is a characteristic feature of SSPE and MIBE. To determine whether the M gene is the preferred target of hypermutation, an additional transcriptional unit containing a humanized Renilla reniformis green fluorescent protein (hrGFP) gene was introduced into the IC323 MV genome, and nude mice were inoculated intracerebrally with the virus. Biased hypermutation occurred in the M gene and also in the hrGFP gene when it was inserted between the leader and the N gene, but not between the El and L gene. These results indicate that biased hypermutation is usually found in a gene whose function is not essential for viral proliferation in the brain and that the location of a gene in the MV genome can affect its mutational frequency. (C) 2014 Elsevier Inc. All rights reserved.
- Link information
- ID information
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- DOI : 10.1016/j.virol.2014.05.035
- ISSN : 0042-6822
- Web of Science ID : WOS:000340225100010