論文

2003年11月

Mechanism of up-regulation of human Toll-like receptor 3 secondary to infection of measles virus-attenuated strains

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • M Tanabe
  • ,
  • M Kurita-Taniguchi
  • ,
  • K Takeuchi
  • ,
  • M Takeda
  • ,
  • M Ayata
  • ,
  • H Ogura
  • ,
  • M Matsumoto
  • ,
  • T Seya

311
1
開始ページ
39
終了ページ
48
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2003.09.159
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

PolyI:C, a synthetic double-stranded (ds)RNA, and viruses act on cells to induce IFN-beta which is a key molecule for anti-viral response. Although dsRNA is a virus-specific signature and a ligand for human Toll-like receptor 3 (TLR3), largely uncharacterized multiple pathways associate virus-mediated IFN-beta induction. Here, we demonstrated that laboratory-adapted but not wild-type strains of measles virus (MV) up-regulated TLR3 expression both in dendritic cells and epithelial cell line A549. The kinetics experiments with the laboratory MV strain revealed that TLR3 was induced late compared to IFN-beta and required new protein synthesis. Furthermore, neutralizing antibodies against IFN-beta or IFNAR (Interferon-alpha/beta receptor) suppressed MV-induced TLR3 induction, indicating that type I IFN, IFN-alpha/beta, is critical for MV-mediated TLR3 induction. Yet, a recently identified virus-inducible IFN, the IFN-lambda, did not contribute to TLR3 expression. A virus-responsive element that up-regulates TLR3 was identified in the TLR3-promoter region by reporter gene experiments. The ISRE, a recently reported site for IFN-beta induction, but not STAT binding site, located around -30 bp of TLR3 promoter responded to MV to induce TLR3 expression. This further indicates the importance of type I IFN for TLR3 up-regulation in the case of viral infection. In HeLa and MRC5 cells, augmented production of IFN-beta was observed in response to dsRNA when TLR3 had been induced beforehand. Thus, the MV-induced expression of TLR3 may reflect amplified IFN production that plays a part in host defense to viral infection. (C) 2003 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 84

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2003.09.159
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000186318200007&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2003.09.159
  • ISSN : 0006-291X
  • Web of Science ID : WOS:000186318200007

エクスポート
BibTeX RIS