TAKAGI Hiroshi

Last updated: Aug 27, 2019 at 08:27

Name	TAKAGI Hiroshi
Affiliation	Nara Institute of Science and Technology
Section	Graduate School of Biological Sciences
Job title	Professor
Degree	Ph.D.(The University of Tokyo)

Research Interests

Applied molecular microbiology(3) , Stress microbiology(1)

Research Areas

Boundary agriculture / Applied molecular and cellular biology /
Agricultural chemistry / Applied biochemistry /
Agricultural chemistry / Applied microbiology /

Academic & Professional Experience

Plain Text

Apr 2006

Today

Professor, Nara Institute of Science and Technology

Mar 1986

Sep 1986

Visiting Research Associate, Department of Biochemistry, State University of New York at Stony Brook

Education

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1982

Graduate School, Division of Agriculture, Nagoya University

1980

Faculty of Agriculture, Shizuoka University

Published Papers

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A novel mitochondrial serine O-acetyltransferase, OpSAT1, plays a critical role in sulfur metabolism in the thermotolerant methylotrophic yeast Ogataea parapolymorpha.

J. Y. Yeon, S. J. Yoo, H. Takagi, H. A. Kang

Scientific Reports 8 2377 2018 [Refereed]

Hyperosmotic stress tolerance of transcription activator Msn2-Over expression strain and proline-NO synthesis strain of Saccharomyces cerevisiae in very high gravity bioethanol fermentation

D. A. Nur’utami, L. Haditjaroko, H. Takagi, K. Syamsu

Pakistan Journal of Biotechnology 14 135-139 2017 [Refereed]

Enhanced sodium acetate tolerance in Saccharomyces cerevisiae by the Thr255Ala mutation of the ubiquitin ligase Rsp5

A. Watcharawipas, D. Watanabe, H. Takagi

FEMS Yeast Research 17 fox083 2017 [Refereed]

A unique intracellular compartment formed during the oligotrophic growth of Rhodococcus erythropolis N9T-4

Applied Microbiology and Biotechnology 101 331-340 2017 [Refereed]

Awa1p on the cell surface of sake yeast inhibits biofilm formation and the co-aggregation between sake yeasts and Lactobacillus plantarum ML11-11.

Hirayama S, Shimizu M, Tsuchiya N, Furukawa S, Watanabe D, Shimoi H, Takagi H, Ogihara H, Morinaga Y

Journal of bioscience and bioengineering Nov 2014 [Refereed]

Isolation and functional analysis of yeast ubiquitin ligase Rsp5 variants that alleviate the toxicity of human α-synuclein.

Wijayanti I, Watanabe D, Oshiro S, Takagi H

Journal of biochemistry Nov 2014 [Refereed]

The transcriptional activator Pog1 controls cell cycle, and its phosphorylated form is downregulated by the ubiquitin ligase Dma2 in Saccharomyces cerevisiae.

Oshiro S, Takagi H

FEMS yeast research 14 1015-1027 Nov 2014 [Refereed]

Cloning and heterologous expression of the ftfCNC-2(1) gene from Weissella confusa MBFCNC-2(1) as an extracellular active fructansucrase in Bacillus subtilis.

Malik A, Hapsari MT, Ohtsu I, Ishikawa S, Takagi H

Journal of bioscience and bioengineering Oct 2014 [Refereed]

Involvement of the yciW gene in l-cysteine and l-methionine metabolism in Escherichia coli.

Kawano Y, Ohtsu I, Tamakoshi A, Shiroyama M, Tsuruoka A, Saiki K, Takumi K, Nonaka G, Nakanishi T, Hishiki T, Suematsu M, Takagi H

Journal of bioscience and bioengineering Sep 2014 [Refereed]

Quality control of plasma membrane proteins by Saccharomyces cerevisiae Nedd4-like ubiquitin ligase Rsp5p under environmental stress conditions.

Shiga T, Yoshida N, Shimizu Y, Suzuki E, Sasaki T, Watanabe D, Takagi H

Eukaryotic cell 13 1191-1199 Sep 2014 [Refereed]

Enhancement of l-cysteine production by disruption of yciW in Escherichia coli.

Kawano Y, Ohtsu I, Takumi K, Tamakoshi A, Nonaka G, Funahashi E, Ihara M, Takagi H

Journal of bioscience and bioengineering Aug 2014 [Refereed]

Isolation and characterization of awamori yeast mutants with l-leucine accumulation that overproduce isoamyl alcohol.

Takagi H, Hashida K, Watanabe D, Nasuno R, Ohashi M, Iha T, Nezuo M, Tsukahara M

Journal of bioscience and bioengineering Jul 2014 [Refereed]

Calcineurin inhibitors suppress the high-temperature stress sensitivity of the yeast ubiquitin ligase Rsp5 mutant: a new method of screening for calcineurin inhibitors.

Uesugi S, Watanabe D, Kitajima M, Watanabe R, Kawamura Y, Ohnishi M, Takagi H, Kimura K

FEMS yeast research 14 567-574 Jun 2014 [Refereed]

Mitochondrial metabolism and stress response of yeast: Applications in fermentation technologies.

Kitagaki H, Takagi H

Journal of bioscience and bioengineering 117 383-393 Apr 2014 [Refereed]

Utilization of atmospheric ammonia by an extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4.

Yoshida N, Inaba S, Takagi H

Journal of bioscience and bioengineering 117 28-32 Jan 2014 [Refereed]

Nitric Oxide-Mediated Antioxidative Mechanism in Yeast through the Activation of the Transcription Factor Mac1.

Nasuno R, Aitoku M, Manago Y, Nishimura A, Sasano Y, Takagi H

PloS one 9 e113788 2014 [Refereed]

An organic acid-tolerant HAA1-overexpression mutant of an industrial bioethanol strain of Saccharomyces cerevisiae and its application to the production of bioethanol from sugarcane molasses.

Inaba T, Watanabe D, Yoshiyama Y, Tanaka K, Ogawa J, Takagi H, Shimoi H, Shima J

AMB Express 3 74 Dec 2013 [Refereed]

Characterization of γ-glutamyl kinase mutants from Saccharomyces cerevisiae.

Tatehashi Y, Takagi H

Journal of bioscience and bioengineering 116 576-579 Nov 2013 [Refereed]

Rim15p-mediated regulation of sucrose utilization during molasses fermentation using Saccharomyces cerevisiae strain PE-2.

Inai T, Watanabe D, Zhou Y, Fukada R, Akao T, Shima J, Takagi H, Shimoi H

Journal of bioscience and bioengineering 116 591-594 Nov 2013 [Refereed]

Improvement of fermentation ability under baking-associated stress conditions by altering the POG1 gene expression in baker's yeast.

Sasano Y, Haitani Y, Hashida K, Oshiro S, Shima J, Takagi H

International journal of food microbiology 165 241-245 Aug 2013 [Refereed]

Properties, metabolisms, and applications of (L)-proline analogues.

Bach TM, Takagi H

Applied microbiology and biotechnology 97 6623-6634 Aug 2013 [Refereed]

Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism.

Nasuno R, Hirano Y, Itoh T, Hakoshima T, Hibi T, Takagi H

Proceedings of the National Academy of Sciences of the United States of America 110 11821-11826 Jul 2013 [Refereed]

Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1.

Sasaki T, Takagi H

Genes to cells : devoted to molecular & cellular mechanisms 18 459-475 Jun 2013 [Refereed]

The flavoprotein Tah18-dependent NO synthesis confers high-temperature stress tolerance on yeast cells.

Nishimura A, Kawahara N, Takagi H

Biochemical and biophysical research communications 430 137-143 Jan 2013 [Refereed]

Microbial production of N-acetyl cis-4-hydroxy-L-proline by coexpression of the Rhizobium L-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1.

Bach TM, Hara R, Kino K, Ohtsu I, Yoshida N, Takagi H

Applied microbiology and biotechnology 97 247-257 Jan 2013 [Refereed]

Production of N-acetyl cis-4-hydroxy-L-proline by the yeast N-acetyltransferase Mpr1.

Hoa BT, Hibi T, Nasuno R, Matsuo G, Sasano Y, Takagi H

Journal of bioscience and bioengineering 114 160-165 Aug 2012 [Refereed]

The proline metabolism intermediate Δ1-pyrroline-5-carboxylate directly inhibits the mitochondrial respiration in budding yeast.

Nishimura A, Nasuno R, Takagi H

FEBS letters 586 2411-2416 Jul 2012 [Refereed]

Carbon monoxide utilization of an extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4.

Yano T, Yoshida N, Takagi H

Journal of bioscience and bioengineering 114 53-55 Jul 2012 [Refereed]

Enhancement of thioredoxin/glutaredoxin-mediated L-cysteine synthesis from S-sulfocysteine increases L-cysteine production in Escherichia coli.

Nakatani T, Ohtsu I, Nonaka G, Wiriyathanawudhiwong N, Morigasaki S, Takagi H

Microbial cell factories 11 62 May 2012 [Refereed]

Simultaneous accumulation of proline and trehalose in industrial baker's yeast enhances fermentation ability in frozen dough.

Sasano Y, Haitani Y, Hashida K, Ohtsu I, Shima J, Takagi H

Journal of bioscience and bioengineering 113 592-595 May 2012 [Refereed]

Enhancement of the proline and nitric oxide synthetic pathway improves fermentation ability under multiple baking-associated stress conditions in industrial baker's yeast.

Sasano Y, Haitani Y, Hashida K, Ohtsu I, Shima J, Takagi H

Microbial cell factories 11 40 Apr 2012 [Refereed]

Overexpression of the yeast transcription activator Msn2 confers furfural resistance and increases the initial fermentation rate in ethanol production.

Sasano Y, Watanabe D, Ukibe K, Inai T, Ohtsu I, Shimoi H, Takagi H

Journal of bioscience and bioengineering 113 451-455 Apr 2012 [Refereed]

Proline accumulation in baker's yeast enhances high-sucrose stress tolerance and fermentation ability in sweet dough.

Sasano Y, Haitani Y, Ohtsu I, Shima J, Takagi H

International journal of food microbiology 152 40-43 Jan 2012 [Refereed]

Overexpression of the transcription activator Msn2 enhances the fermentation ability of industrial baker's yeast in frozen dough.

Sasano Y, Haitani Y, Hashida K, Ohtsu I, Shima J, Takagi H

Bioscience, biotechnology, and biochemistry 76 624-627 2012 [Refereed]

Functional analysis of the C-terminal region of γ-glutamyl kinase of Saccharomyces cerevisiae.

Kaino T, Tasaka Y, Tatehashi Y, Takagi H

Bioscience, biotechnology, and biochemistry 76 454-461 2012 [Refereed]

Sake yeast strains have difficulty in entering a quiescent state after cell growth cessation.

Urbanczyk H, Noguchi C, Wu H, Watanabe D, Akao T, Takagi H, Shimoi H

Journal of bioscience and bioengineering 112 44-48 Jul 2011 [Refereed]

Gene expression analysis of methylotrophic oxidoreductases involved in the oligotrophic growth of Rhodococcus erythropolis N9T-4.

Yoshida N, Hayasaki T, Takagi H

Bioscience, biotechnology, and biochemistry 75 123-127 2011 [Refereed]

An antioxidative mechanism mediated by the yeast N-acetyltransferase Mpr1: oxidative stress-induced arginine synthesis and its physiological role.

Nishimura A, Kotani T, Sasano Y, Takagi H

FEMS yeast research 10 687-698 Sep 2010 [Refereed]

The L-cysteine/L-cystine shuttle system provides reducing equivalents to the periplasm in Escherichia coli.

Ohtsu I, Wiriyathanawudhiwong N, Morigasaki S, Nakatani T, Kadokura H, Takagi H

The Journal of biological chemistry 285 17479-17487 Jun 2010 [Refereed]

Multicopy suppression of oxidant-sensitive eos1 mutation by IZH2 in Saccharomyces cerevisiae and the involvement of Eos1 in zinc homeostasis.

Nakamura T, Takahashi S, Takagi H, Shima J

FEMS yeast research 10 259-269 May 2010 [Refereed]

Antioxidant N-acetyltransferase Mpr1/2 of industrial baker's yeast enhances fermentation ability after air-drying stress in bread dough.

Sasano Y, Takahashi S, Shima J, Takagi H

International journal of food microbiology 138 181-185 Mar 2010 [Refereed]

Metabolic engineering of Saccharomyces cerevisiae for astaxanthin production and oxidative stress tolerance.

Ukibe K, Hashida K, Yoshida N, Takagi H

Applied and environmental microbiology 75 7205-7211 Nov 2009 [Refereed]

MPR1 as a novel selection marker in Saccharomyces cerevisiae.

Ogawa-Mitsuhashi K, Sagane K, Kuromitsu J, Takagi H, Tsukahara K

Yeast (Chichester, England) 26 587-593 Nov 2009 [Refereed]

Insufficiency of copper ion homeostasis causes freeze-thaw injury of yeast cells as revealed by indirect gene expression analysis.

Takahashi S, Ando A, Takagi H, Shima J

Applied and environmental microbiology 75 6706-6711 Nov 2009 [Refereed]

[Novel yeast acetyltransferase Mpr1 regulates reactive oxygen species mediated by proline metabolism].

Takagi H

Seikagaku. The Journal of Japanese Biochemical Society 81 891-895 Oct 2009 [Refereed]

A functional analysis of the yeast ubiquitin ligase Rsp5: the involvement of the ubiquitin-conjugating enzyme Ubc4 and poly-ubiquitination in ethanol-induced down-regulation of targeted proteins.

Hiraishi H, Okada M, Ohtsu I, Takagi H

Bioscience, biotechnology, and biochemistry 73 2268-2273 Oct 2009 [Refereed]

[Highly unusual non-ribosomal peptide synthetase producing an amino-acid homopolymer].

Hamano Y, Yamanaka K, Maruyama C, Takagi H

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 54 1382-1388 Sep 2009 [Refereed]

Proline as a stress protectant in the yeast Saccharomyces cerevisiae: effects of trehalose and PRO1 gene expression on stress tolerance.

Kaino T, Takagi H

Bioscience, biotechnology, and biochemistry 73 2131-2135 Sep 2009 [Refereed]

The yeast ubiquitin ligase Rsp5 downregulates the alpha subunit of nascent polypeptide-associated complex Egd2 under stress conditions.

Hiraishi H, Shimada T, Ohtsu I, Sato TA, Takagi H

The FEBS journal 276 5287-5297 Sep 2009 [Refereed]

Disruption of ubiquitin-related genes in laboratory yeast strains enhances ethanol production during sake brewing.

Wu H, Watanabe T, Araki Y, Kitagaki H, Akao T, Takagi H, Shimoi H

Journal of bioscience and bioengineering 107 636-640 Jun 2009 [Refereed]

Engineering of the yeast antioxidant enzyme Mpr1 for enhanced activity and stability.

Iinoya K, Kotani T, Sasano Y, Takagi H

Biotechnology and bioengineering 103 341-352 Jun 2009 [Refereed]

Stress-tolerance of baker's-yeast (Saccharomyces cerevisiae) cells: stress-protective molecules and genes involved in stress tolerance.

Shima J, Takagi H

Biotechnology and applied biochemistry 53 155-164 May 2009 [Refereed]

Effects of ice-seeding temperature and intracellular trehalose contents on survival of frozen Saccharomyces cerevisiae cells.

Nakamura T, Takagi H, Shima J

Cryobiology 58 170-174 Apr 2009 [Refereed]

Crystallization and preliminary crystallographic analysis of N-acetyltransferase Mpr1 from Saccharomyces cerevisiae.

Hibi T, Yamamoto H, Nakamura G, Takagi H

Acta crystallographica. Section F, Structural biology and crystallization communications 65 169-172 Feb 2009 [Refereed]

Engineering of the yeast ubiquitin ligase Rsp5: isolation of a new variant that induces constitutive inactivation of the general amino acid permease Gap1.

Haitani Y, Nakata M, Sasaki T, Uchida A, Takagi H

FEMS yeast research 9 73-86 Feb 2009 [Refereed]

Ethanol stress stimulates the Ca2+-mediated calcineurin/Crz1 pathway in Saccharomyces cerevisiae.

Araki Y, Wu H, Kitagaki H, Akao T, Takagi H, Shimoi H

Journal of bioscience and bioengineering 107 1-6 Jan 2009 [Refereed]

The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli.

Wiriyathanawudhiwong N, Ohtsu I, Li ZD, Mori H, Takagi H

Applied microbiology and biotechnology 81 903-913 Jan 2009 [Refereed]

Epsilon-poly-L-lysine dispersity is controlled by a highly unusual nonribosomal peptide synthetase.

Yamanaka K, Maruyama C, Takagi H, Hamano Y

Nature chemical biology 4 766-772 Dec 2008 [Refereed]

Proline as a stress protectant in yeast: physiological functions, metabolic regulations, and biotechnological applications.

Takagi H

Applied microbiology and biotechnology 81 211-223 Nov 2008 [Refereed]

Changes in gene expression of commercial baker's yeast during an air-drying process that simulates dried yeast production.

Nakamura T, Mizukami-Murata S, Ando A, Murata Y, Takagi H, Shima J

Journal of bioscience and bioengineering 106 405-408 Oct 2008 [Refereed]

Efficient screening for astaxanthin-overproducing mutants of the yeast Xanthophyllomyces dendrorhous by flow cytometry.

Ukibe K, Katsuragi T, Tani Y, Takagi H

FEMS microbiology letters 286 241-248 Sep 2008 [Refereed]

Self-cloning baker's yeasts that accumulate proline enhance freeze tolerance in doughs.

Kaino T, Tateiwa T, Mizukami-Murata S, Shima J, Takagi H

Applied and environmental microbiology 74 5845-5849 Sep 2008 [Refereed]

Identification of amino acid residues essential for the yeast N-acetyltransferase Mpr1 activity by site-directed mutagenesis.

Kotani T, Takagi H

FEMS yeast research 8 607-614 Jun 2008 [Refereed]

Gene expression profiles and intracellular contents of stress protectants in Saccharomyces cerevisiae under ethanol and sorbitol stresses.

Kaino T, Takagi H

Applied microbiology and biotechnology 79 273-283 May 2008 [Refereed]

[Proline functions and its metabolic regulations in yeast: relevance to stress adaptation-strategy].

Takagi H

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme 53 249-255 Mar 2008 [Refereed]

Possible roles of vacuolar H+-ATPase and mitochondrial function in tolerance to air-drying stress revealed by genome-wide screening of Saccharomyces cerevisiae deletion strains.

Shima J, Ando A, Takagi H

Yeast (Chichester, England) 25 179-190 Mar 2008 [Refereed]

Distribution of L-azetidine-2-carboxylate N-acetyltransferase in yeast.

Wada M, Okabe K, Kataoka M, Shimizu S, Yokota A, Takagi H

Bioscience, biotechnology, and biochemistry 72 582-586 Feb 2008 [Refereed]

Rsp5 is required for the nuclear export of mRNA of HSF1 and MSN2/4 under stress conditions in Saccharomyces cerevisiae.

Haitani Y, Takagi H

Genes to cells : devoted to molecular & cellular mechanisms 13 105-116 Feb 2008 [Refereed]

Overexpression of two transcriptional factors, Kin28 and Pog1, suppresses the stress sensitivity caused by the rsp5 mutation in Saccharomyces cerevisiae.

Demae M, Murata Y, Hisano M, Haitani Y, Shima J, Takagi H

FEMS microbiology letters 277 70-78 Dec 2007 [Refereed]

Functional analysis of genes encoding putative oxidoreductases in Aspergillus oryzae, which are similar to fungal fructosyl-amino acid oxidase.

Yoshida N, Akazawa S, Karino T, Ishida H, Hata Y, Katsuragi T, Tani Y, Takagi H

Journal of bioscience and bioengineering 104 424-427 Nov 2007 [Refereed]

Screening of carbon dioxide-requiring extreme oligotrophs from soil.

Yoshida N, Ohhata N, Yoshino Y, Katsuragi T, Tani Y, Takagi H

Bioscience, biotechnology, and biochemistry 71 2830-2832 Nov 2007 [Refereed]

Functional genomics of commercial baker's yeasts that have different abilities for sugar utilization and high-sucrose tolerance under different sugar conditions.

Tanaka-Tsuno F, Mizukami-Murata S, Murata Y, Nakamura T, Ando A, Takagi H, Shima J

Yeast (Chichester, England) 24 901-911 Oct 2007 [Refereed]

An extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4, isolated from crude oil.

Ohhata N, Yoshida N, Egami H, Katsuragi T, Tani Y, Takagi H

Journal of bacteriology 189 6824-6831 Oct 2007 [Refereed]

N-Acetyltransferase Mpr1 confers ethanol tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species.

Du X, Takagi H

Applied microbiology and biotechnology 75 1343-1351 Jul 2007 [Refereed]

Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance.

Sekine T, Kawaguchi A, Hamano Y, Takagi H

Applied and environmental microbiology 73 4011-4019 Jun 2007 [Refereed]

Construction and analysis of self-cloning sake yeasts that accumulate proline.

Takagi H, Matsui F, Kawaguchi A, Wu H, Shimoi H, Kubo Y

Journal of bioscience and bioengineering 103 377-380 Apr 2007 [Refereed]

Identification and classification of genes required for tolerance to freeze-thaw stress revealed by genome-wide screening of Saccharomyces cerevisiae deletion strains.

Ando A, Nakamura T, Murata Y, Takagi H, Shima J

FEMS yeast research 7 244-253 Mar 2007 [Refereed]

EOS1, whose deletion confers sensitivity to oxidative stress, is involved in N-glycosylation in Saccharomyces cerevisiae.

Nakamura T, Ando A, Takagi H, Shima J

Biochemical and biophysical research communications 353 293-298 Feb 2007 [Refereed]

Functional genomic analysis of commercial baker's yeast during initial stages of model dough-fermentation.

Tanaka F, Ando A, Nakamura T, Takagi H, Shima J

Food microbiology 23 717-728 Dec 2006 [Refereed]

Enhancement of stress tolerance in Saccharomyces cerevisiae by overexpression of ubiquitin ligase Rsp5 and ubiquitin-conjugating enzymes.

Hiraishi H, Mochizuki M, Takagi H

Bioscience, biotechnology, and biochemistry 70 2762-2765 Nov 2006 [Refereed]

Metabolic pathways and biotechnological production of L-cysteine.

Wada M, Takagi H

Applied microbiology and biotechnology 73 48-54 Nov 2006 [Refereed]

Global gene expression analysis of yeast cells during sake brewing.

Wu H, Zheng X, Araki Y, Sahara H, Takagi H, Shimoi H

Applied and environmental microbiology 72 7353-7358 Nov 2006 [Refereed]

Effect of drug transporter genes on cysteine export and overproduction in Escherichia coli.

Yamada S, Awano N, Inubushi K, Maeda E, Nakamori S, Nishino K, Yamaguchi A, Takagi H

Applied and environmental microbiology 72 4735-4742 Jul 2006 [Refereed]

Rsp5 regulates expression of stress proteins via post-translational modification of Hsf1 and Msn4 in Saccharomyces cerevisiae.

Haitani Y, Shimoi H, Takagi H

FEBS letters 580 3433-3438 Jun 2006 [Refereed]

A novel enzyme conferring streptothricin resistance alters the toxicity of streptothricin D from broad-spectrum to bacteria-specific.

Hamano Y, Matsuura N, Kitamura M, Takagi H

The Journal of biological chemistry 281 16842-16848 Jun 2006 [Refereed]

Yeast cell death caused by mutation of the OST2 gene encoding the epsilon-subunit of Saccharomyces cerevisiae oligosaccharyltransferase.

Sugiura M, Takagi H

Bioscience, biotechnology, and biochemistry 70 1234-1241 May 2006 [Refereed]

Identification and classification of genes required for tolerance to high-sucrose stress revealed by genome-wide screening of Saccharomyces cerevisiae.

Ando A, Tanaka F, Murata Y, Takagi H, Shima J

FEMS yeast research 6 249-267 Mar 2006 [Refereed]

Functional analysis of L-serine O-acetyltransferase from Corynebacterium glutamicum.

Haitani Y, Awano N, Yamazaki M, Wada M, Nakamori S, Takagi H

FEMS microbiology letters 255 156-163 Feb 2006 [Refereed]

Effect of L-proline on sake brewing and ethanol stress in Saccharomyces cerevisiae.

Takagi H, Takaoka M, Kawaguchi A, Kubo Y

Applied and environmental microbiology 71 8656-8662 Dec 2005 [Refereed]

Vacuolar functions are involved in stress-protective effect of intracellular proline in Saccharomyces cerevisiae.

Matsuura K, Takagi H

Journal of bioscience and bioengineering 100 538-544 Nov 2005 [Refereed]

N-acetyltransferase Mpr1 confers freeze tolerance on Saccharomyces cerevisiae by reducing reactive oxygen species.

Du X, Takagi H

Journal of biochemistry 138 391-397 Oct 2005 [Refereed]

A sericin-derived peptide protects sf9 insect cells from death caused by acute serum deprivation.

Takahashi M, Tsujimoto K, Kato Y, Yamada H, Takagi H, Nakamori S

Biotechnology letters 27 893-897 Jul 2005 [Refereed]

Identification and functional analysis of Escherichia coli cysteine desulfhydrases.

Awano N, Wada M, Mori H, Nakamori S, Takagi H

Applied and environmental microbiology 71 4149-4152 Jul 2005 [Refereed]

Development of gene delivery systems for the epsilon-poly-L-lysine producer, Streptomyces albulus.

Hamano Y, Nicchu I, Hoshino Y, Kawai T, Nakamori S, Takagi H

Journal of bioscience and bioengineering 99 636-641 Jun 2005 [Refereed]

A novel metal-activated L-serine O-acetyltransferase from Thermus thermophilus HB8.

Kobayashi S, Masui R, Yokoyama S, Kuramitsu S, Takagi H

Journal of biochemistry 136 629-634 Nov 2004 [Refereed]

The production of recombinant APRP, an alkaline protease derived from Bacillus pumilus TYO-67, by in vitro refolding of pro-enzyme fixed on a solid surface.

Takahashi M, Sekine T, Kuba N, Nakamori S, Yasuda M, Takagi H

Journal of biochemistry 136 549-556 Oct 2004 [Refereed]

Overexpression and characterization of an aminoglycoside 6'-N-acetyltransferase with broad specificity from an epsilon-poly-L-lysine producer, Streptomyces albulus IFO14147.

Hamano Y, Hoshino Y, Nakamori S, Takagi H

Journal of biochemistry 136 517-524 Oct 2004 [Refereed]

Role of the yeast acetyltransferase Mpr1 in oxidative stress: regulation of oxygen reactive species caused by a toxic proline catabolism intermediate.

Nomura M, Takagi H

Proceedings of the National Academy of Sciences of the United States of America 101 12616-12621 Aug 2004 [Refereed]

Characterization of end4+, a gene required for endocytosis in Schizosaccharomyces pombe.

Iwaki T, Tanaka N, Takagi H, Giga-Hama Y, Takegawa K

Yeast (Chichester, England) 21 867-881 Jul 2004 [Refereed]

Purification and characterization of O-Acetylserine sulfhydrylase of Corynebacterium glutamicum.

Wada M, Awano N, Yamazawa H, Takagi H, Nakamori S

Bioscience, biotechnology, and biochemistry 68 1581-1583 Jul 2004 [Refereed]

Misc

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Chemical Synthesis of the gene for Microbial Transglutaminase from ┣DBStreptoverticillium(/)-┫DB and its Expression in ┣DBEscherichia coli(/)-┫DB(jointly worked)

Bioscience, Biotechnology, and Biochemistry 58(1) 88 1994

Subtilisin

Protein, Nucleic Acid and Enzyme 37(3) 303 1992

Protein Engineering on Subtilisin

International Journal of Biochemistry 25(3) 307-312 1993

Effects of an Alkaline Elastase from an Alkalophilic ┣DBBacillus(/)-┫DB Strain on the Tenderization of Beef Meat(jointly worked)

Journal of Agricultural and Food Chemistry 40(12) 2364-2368 1992

The Effect of Amino Acid Deletion in Subtilisin E, Based on Structural Comparison with a Microbial Alkaline Elastase, on its Substrate Specificity and Catalysis(jointly worked)

Journal of Biochemistry 111(5) 584 1992

Books etc

Plain Text

Subtilisin Enzymes Practical Protein Engineering(jointly worked)

Advances in Experimental Medicine and Biology Plenum Press 1996

Protein-Science and Engineering

1999

Association Memberships

JAPANESE SOCIETY FOR EXTREMOPHILES(45)

Works

Plain Text

Molecular Breeding of Sulfur-containing Amino acid Producing Bacteria

1999

Research Grants & Projects

Plain Text

Molecular analysis of stress-torelant mechanisms and theri applications in the yeast Schhacromyces cerevisiae

Molecular Breeding of Sulfur-containing Amino acid Producing Bacteria

Improvement of Enzyme Function by Protein Engineering