論文

査読有り
2015年6月

Activity of ERK regulates mucin 3 expression and is involved in undifferentiated Caco-2 cell death induced by 3-oxo-C-12-homoserine lactone

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
  • Hidehisa Shimizu
  • Nanako Baba
  • Takuma Nose
  • Ryoko Taguchi
  • Shinya Tanaka
  • Ga-Hyun Joe
  • Hideaki Maseda
  • Nobuhiko Nomura
  • Masahito Hagio
  • Ja-Young Lee
  • Satoru Fukiya
  • Atsushi Yokota
  • Satoshi Ishizuka
  • Hitoshi Miyazaki
  • 全て表示

79
6
開始ページ
937
終了ページ
942
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2015.1006570
出版者・発行元
TAYLOR & FRANCIS LTD

The signal molecule, 3-oxo-C-12-homoserine lactone (3-oxo-C-12-HSL), is similar to a mammalian hormone in bacteria. Although most studies have examined the effects of high 3-oxo-C-12-HSL concentrations (>200 mu M) on mammalian cellular functions because ~600 mu M 3-oxo-C-12-HSL can be secreted in biofilms of Pseudomonas aeruginosa grown in vitro, we previously showed that a low 3-oxo-C-12-HSL concentration (30 mu M) induces the apoptosis of undifferentiated Caco-2 cells through suppressing Akt activity. Here, we found that a low concentration of 3-oxo-C-12-HSL-activated ERK1/2 in undifferentiated Caco-2 cells. Incubating cells with the ERK pathway inhibitor U0126 for 30min alleviated the mucin 3 (MUC3) expression suppressed by 3-oxo-C-12-HSL, and the upregulation of MUC3 expression induced by a 48-h incubation with U0126-reduced cell death. Thus, altered MUC3 expression caused by long-term attenuated ERK1/2 activity might correlate with the death of undifferentiated Caco-2 cells induced by 3-oxo-C-12-HSL.

リンク情報
DOI
https://doi.org/10.1080/09168451.2015.1006570
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25774422
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000356239400010&DestApp=WOS_CPL
ID情報
  • DOI : 10.1080/09168451.2015.1006570
  • ISSN : 0916-8451
  • eISSN : 1347-6947
  • PubMed ID : 25774422
  • Web of Science ID : WOS:000356239400010

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