We report on the performance of a micro-Time Projection Chamber(TPC) with a micro-pixel chamber(mu-PIC) readout for a time-resolved neutron imaging detector. We developed a mu-TPC with a mu-PIC, which is a type of micro pattern gas detector, and detected three-dimensional (3-D) tracks and the Bragg curves of protons with energies of around 1 MeV. Based on this technique we pointed out that the 3-D measurement of the decay pattern of He-3 (n, p (573 keV)) H-3 (191 keV) reaction would enable us to distinguish the proton and triton using the energy loss information (Nucl. Instr. and Meth. 517 (2004) 219). Here we present the first result of the 3-D measurement of the decay pattern of He-3 reaction with the energy loss information using the mu-TPC. (C) 2004 Elsevier B.V. All rights reserved.