論文

査読有り
2001年

Oligoclonally expanding γδ T lymphocytes induce IgA switching in IgA nephropathy

Clinical and Experimental Immunology
  • Shin-Ichi Toyabe
  • ,
  • W. Harada
  • ,
  • M. Uchiyama

124
1
開始ページ
110
終了ページ
117
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1046/j.1365-2249.2001.01494.x

The aetiology of IgA nephropathy (IgAN) is closely related with abnormality of mucosal immunity. We investigated the roles of γδ T cells in the regulation of IgA production by B cells in IgAN patients. The proportion of γδ T cells in peripheral blood mononuclear cells (PBMNC) was higher in IgAN patients than in the controls and was found to be correlated with the proportion of surface IgA-positive (sIgA+) B cells, which are precursors of IgA-secreting plasma cells. After in vitro PWM stimulation, sIgA expression on B cells and IgA production were significantly enhanced in PBMNC obtained from IgAN patients, whereas the enhancements were abolished by removal of γδ T cells from the PBMNC. Purified γδ T cells from IgAN patients induced surface IgA expression on naïve sIgD+ B cells more effectively than did αβ T cells. Moreover, stimulated γδ T cells from IgAN patients produced a larger amount of TGF-β1, which is one of the main cytokines that induces IgA class switching on B cells, as compared with αβ T cells and control γδ T cells. The expanded γδ T cells from IgAN patients exclusively expressed Vγ9, and the nucleotide sequences of junctional regions of Vγ9 showed very limited TCR diversities. It was therefore concluded that γδ T cells, which are expanded in response to specific antigens, enhance IgA class switching on B cells in IgAN patients.

リンク情報
DOI
https://doi.org/10.1046/j.1365-2249.2001.01494.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/11359449
ID情報
  • DOI : 10.1046/j.1365-2249.2001.01494.x
  • ISSN : 0009-9104
  • PubMed ID : 11359449
  • SCOPUS ID : 0034744078

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