1997年3月
Highly efficient system of plant regeneration from protoplasts of grapevine (Vitis vinifera L) through somatic embryogenesis by using embryogenic callus culture and activated charcoal
PLANT SCIENCE
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- 巻
- 123
- 号
- 1-2
- 開始ページ
- 151
- 終了ページ
- 157
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/S0168-9452(96)04557-8
- 出版者・発行元
- ELSEVIER SCI IRELAND LTD
A simple protocol is described for high frequency plant regeneration from protoplasts isolated from leaf-derived embryogenic calli of grapevine (Vitis vinifera L. cv. Koshusanjaku). The protoplasts successfully divided to form somatic embryos by culturing in gellan gum disc-method in which protoplasts were embedded in 2 g/l gellan gum-solidified Nitsch's medium containing 2.0 mg/l NAA, 0.5 mg/l BA, 0.09 M sucrose and 0.3 M glucose at a density of 1 x 10(5) protoplasts/ml. For the continuous growth of the colonies without browning, it was essential to add 0.3% (w/v) AC in the liquid reservoir medium from the beginning of the culture. In this culture condition, protoplasts started to divide after IO days of culture and grew into torpedo embryos 4 months after initiation of culture. The torpedo embryos thus obtained germinated normally by transferring onto 2 g/l gellan gum-solidified PGR-free Nitsch's medium containing 30 g/l sucrose. The regenerated plants were successfully transferred to the greenhouse and showed normal morphology. (C) 1997 Elsevier Science Ireland Ltd.
- リンク情報
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- DOI
- https://doi.org/10.1016/S0168-9452(96)04557-8
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:A1997WN10100017&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0031588964&origin=inward
- ID情報
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- DOI : 10.1016/S0168-9452(96)04557-8
- ISSN : 0168-9452
- Web of Science ID : WOS:A1997WN10100017