2005年
Agrobacterium-mediated production of transgenic plants in tricyrtis hirta (Liliaceae)
Acta Horticulturae
- ,
- ,
- 巻
- 673
- 号
- 開始ページ
- 415
- 終了ページ
- 419
- 記述言語
- 英語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.17660/ActaHortic.2005.673.52
- 出版者・発行元
- International Society for Horticultural Science
A system for producing transgenic plants has been developed for the Liliaceous geophyte Tricyrtis hirta via Agrobacterium-mediated transformation. Tepal-derived embryogenic calluses were co-cultivated with A. tumefaciens EHA101/pIG121Hm, which harbored the binary vector carrying the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing ?-glucuronidase (GUS-intron) genes in the T-DNA region. The duration of co-cultivation and acetosyringone (AS) treatment during co-cultivation affected the frequency of transient expression of the GUS gene: the best result was obtained when embryogenic calluses were co-cultivated for 7 days in the presence of 50 mg l-1 AS. Following co-cultivation, the calluses were transferred to a medium lacking plant growth regulators (PGRs) but containing 40 mg l-1 hygromycin and 300 mg l-1 cefotaxime, on which several hygromycin-resistant (Hygr) somatic embryos were produced 8 weeks after transfer. These embryos were transferred to the same medium but containing lower concentrations of antibiotics (20 mg l -1 hygromycin and 100 mg l-1 cefotaxime) to promote germination. Finally, Hygr embryo-derived plantlets were established on a medium without both PGRs and antibiotics. Most of them were verified to be stable transformants by GUS histochemical assay and PCR analysis.
- リンク情報
- ID情報
-
- DOI : 10.17660/ActaHortic.2005.673.52
- ISSN : 0567-7572
- SCOPUS ID : 33750378818