2016年12月
Generation of a transgenic medaka (Oryzias latipes) strain for visualization of nuclear dynamics in early developmental stages
DEVELOPMENT GROWTH & DIFFERENTIATION
ダウンロード
回数 : 185
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- 巻
- 58
- 号
- 9
- 開始ページ
- 679
- 終了ページ
- 687
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/dgd.12324
- 出版者・発行元
- WILEY-BLACKWELL
In this study, we verified nuclear transport activity of an artificial nuclear localization signal (aNLS) in medaka fish (Oryzias latipes). We generated a transgenic medaka strain expresses the aNLS tagged enhanced green fluorescent protein (EGFP) driven by a medaka beta-actin promoter. The aNLS-EGFP was accumulated in the nuclei of somatic tissues and yolk nuclei of oocytes, but undetectable in the spermatozoa. The fluorescent signal was observed from immediately after fertilization by a maternal contribution. Furthermore, male and female pronuclei were visualized in fertilized eggs, and nuclear dynamics of pronuclear fusion and subsequent cleavage were captured by time-lapse imaging. In contrast, SV40NLS exhibited no activity of nuclear transport in early embryos. In conclusion, the aNLS possesses a strong nuclear localization activity and is a useful probe for fluorescent observation of the pronuclei and nuclei in early developmental stage of medaka.
- リンク情報
- ID情報
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- DOI : 10.1111/dgd.12324
- ISSN : 0012-1592
- eISSN : 1440-169X
- Web of Science ID : WOS:000392727900001