2000年2月
Lysyl-tRNA synthetase of Bacillus stearothermophilus molecular cloning and expression of the gene
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 64
- 号
- 2
- 開始ページ
- 432
- 終了ページ
- 437
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.64.432
- 出版者・発行元
- TAYLOR & FRANCIS LTD
The gene of the lysyl-tRNA synthetase of Bacillus stearothermophilus NCA1503 was cloned and sequenced. The gene consists of 1485 bp nucleotides commencing with an ATG start codon and ending with a TAA stop codon, and encodes a polypeptide of 493 amino acids, The recombinant enzymes were expressed in E. coli using an expression plasmid containing the T7 RNA polymerase/promoter.
- リンク情報
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- DOI
- https://doi.org/10.1271/bbb.64.432
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=200902100282926292
- CiNii Articles
- http://ci.nii.ac.jp/naid/110002679931
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/10737207
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000085778200036&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1271/bbb.64.432
- ISSN : 0916-8451
- eISSN : 1347-6947
- J-Global ID : 200902100282926292
- CiNii Articles ID : 110002679931
- PubMed ID : 10737207
- Web of Science ID : WOS:000085778200036