2012年7月
Stability of acetaldehyde-derived DNA adduct in vitro
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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- 巻
- 423
- 号
- 4
- 開始ページ
- 642
- 終了ページ
- 646
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bbrc.2012.05.158
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Acetaldehyde (AA) derived from alcoholic beverages is a confirmed carcinogen for esophageal and head and neck cancers. AA forms various DNA adducts and is thought to play a crucial role in carcinogenesis. Transient DNA adducts are usually repaired, but the stability of AA-derived DNA adducts has not been elucidated. We investigated the stability of N-2-ethylidene-2'-deoxyguanosine (N-2-ethylidene-dG), a major AA-derived DNA adduct, in cultured cells. First, to determine the optimal concentration of AA for detecting N-2-ethylidene-dG in cell culture, a dose-response study was performed using HL60 cells of the human promyelocytic leukemia cell line. An AA concentration, >= 0.01% (1.8 mM) was required to detect N-2-ethylidene-dG in vitro. We next examined the stability of N-2-ethylidene-dG. After a 1 or 2 h exposure to 0.01% of AA in a tightly sealed bottle, N-2-ethylidene-dG content was measured by sensitive liquid chromatography tandem mass spectrometry immediately, 24 h, and 48 h after exposure. After the 1 h exposure, the mean (+/- SD) N-2-ethylidene-dG contents were 12.1 +/- 1.28, 8.20 +/- 0.64, and 6.70 +/- 0.52 adducts per 10(7) bases at each postexposure time. After the 2 h exposure, N-2-ethylidene-dG content increased to 21.4 +/- 7.50, 10.5 +/- 3.61, and 9.83 +/- 3.90 adducts per 10(7) bases at each postexposure time. The half-life of this adduct was calculated as similar to 35 h in independent experiments. These results indicate that AA exposure from daily alcohol consumption may cause DNA damage and may increase the risk of alcohol-related carcinogenesis. (C) 2012 Elsevier Inc. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.bbrc.2012.05.158
- J-GLOBAL
- https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201202280842938139
- CiNii Articles
- http://ci.nii.ac.jp/naid/120004873686
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22683642
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000307032400005&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1016/j.bbrc.2012.05.158
- ISSN : 0006-291X
- J-Global ID : 201202280842938139
- CiNii Articles ID : 120004873686
- PubMed ID : 22683642
- Web of Science ID : WOS:000307032400005