To develop and apply the findings that vagus nerve stimulation promotes the secretory function of salivary glands and to clarify the effects of parasympathetic electrical stimulation of the autonomic nervous system on tissue blood flow in the salivary glands, masseter muscle, and mandibular periosteum in the oral and maxillary region, we performed vagus nerve stimulation in rodents. The results showed a close relationship between salivary gland tissue blood flow and salivation induced by vagus nerve stimulation, suggesting the existence of a muscarinic receptor-mediated control mechanism of tissue blood flow. Furthermore, the results suggest that vagus nerve stimulation affects tissue blood flow in the periosteum. Further studies are needed to elucidate the mechanism by which vagus nerve stimulation produces ischemia in the masseter muscle and its physiological significance in influencing tissue blood flow in the mandibular periosteum.