A knockdown experiment of Nesprin - 1 using siRNA for osteoblast cell line Saos 2 was performed. By introducing Si - Nesprin 1, it was confirmed by immunofluorescence that nuclear localization of Nesprin decreased. Furthermore, it was confirmed that si-Nesprin 1 suppresses expression at the protein level by the Western blotting method. Therefore, it was confirmed that si-Nesprin 1 was introduced into Saos 2 cells.
Next, culture experiments of knockdown cells using uneven surface structures were performed. As a result, there was no significant difference between the two in the arrangement of the cells. Next, in order to examine the change of Saos 2 in cell polarity in mechanical stress, cell extension experiment with flexer cell unit was performed. However, in Saos 2, no change in polarity to mechanical stress was observed, so Nesprin's involvement was not considered.