2003年8月
Dual effect of saikogenin D: In vitro inhibition of prostaglandin E-2 production and elevation of intracellular free Ca2+ concentration in C6 rat glioma cells
PLANTA MEDICA
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- 巻
- 69
- 号
- 8
- 開始ページ
- 765
- 終了ページ
- 767
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1055/s-2003-42795
- 出版者・発行元
- GEORG THIEME VERLAG KG
To clarify the pharmacological profile of saikogenin D, we examined the effect of saikogenin D on prostaglandin E-2 (PGE(2)) production and intracellular free Ca2+ concentration ([Ca2+](i)) in C6 rat glioma cells. Saikogenin D (1 - 20 muM) inhibited PGE(2) production induced by the Ca2+ ionophore A23187 in a concentration-dependent manner with the IC50 of about 3 muM. Saikogenin D did not affect the conversion of arachidonic acid into PGE(2) in microsomal preparations. On the other hand, saikogenin D elevated [Ca2+](i) in a concentration-dependent manner (10 - 100 muM) with the EC50 value of about 35 muM in the presence or absence of extracellular Ca2+. These results suggest that saikogenin D possesses a dual effect: an inhibition of A23187-induced PGE(2) production without a direct inhibition of cyclooxygenase activity; and an elevation of [Ca2+](i) that is attributed to Ca2+ release from intracellular stores.
- リンク情報
- ID情報
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- DOI : 10.1055/s-2003-42795
- ISSN : 0032-0943
- Web of Science ID : WOS:000185862100015