1999年4月
Extraction and partial characterization of pupal-cuticle-melanizing hormone (PCMH) in the swallowtail butterfly, Papilio xuthus L. (Lepidoptera, Papilionidae)(Developmental Biology) :
Zoological science
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- 巻
- 16
- 号
- 2
- 開始ページ
- 261
- 終了ページ
- 268
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.2108/zsj.16.261
- 出版者・発行元
- 社団法人 日本動物学会
The pupae of Papilio xuthus show green, brown, and orange color polymorphism. The color of the pupal body is determined by a hormone that produces brown coloration, a hormone called pupal-cuticlemelanizing hormone (PCMH) which is secreted from brain-suboesophageal ganglion and prothoracic ganglion (Br-SG-PG) complexes during the pharate pupal stage. PCMH was extracted with 2% NaCl from Br-SG-PG complexes of P. xuthus green pupae. When pharate pupae producing brown pupae (brown-pupaproducers) were ligatured between the thorax and abdomen with cotton thread, the head-thorax complex developed into the brown type and the abdomens into the green type. Ligatured abdomens treated with crude PCMH produced intermediates of green- and brown-pupae (melanization degree of grades 0-3), which was used to assay PCMH-activity. Extracts of Br-SG complexes from Bombyx mori adults also shifted the color of the pupal cuticle toward the brown type in ligatured P. xuthus abdomens of brown-pupa-producers. The molecular weight of the B. mori factor showing PCMH activity (PCMH-active factor) was estimated to be 3,000-4,000 Da by gel filtration. The PCMH-active factor is a hydrophobic peptide (s) that binds to a cation exchange resin at pH 6.9.
- リンク情報
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- DOI
- https://doi.org/10.2108/zsj.16.261
- CiNii Articles
- http://ci.nii.ac.jp/naid/110003370318
- CiNii Books
- http://ci.nii.ac.jp/ncid/AA10545874
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000081915900010&DestApp=WOS_CPL
- URL
- http://www.bioone.org/doi/pdf/10.2108/zsj.16.261 本文へのリンクあり
- ID情報
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- DOI : 10.2108/zsj.16.261
- ISSN : 0289-0003
- CiNii Articles ID : 110003370318
- CiNii Books ID : AA10545874
- Web of Science ID : WOS:000081915900010