2011年3月
Destruction of Amyloid Fibrils of Keratoepithelin Peptides by Laser Irradiation Coupled with Amyloid-specific Thioflavin
JOURNAL OF BIOLOGICAL CHEMISTRY
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 286
- 号
- 12
- 開始ページ
- 10856
- 終了ページ
- 10863
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1074/jbc.M111.222901
- 出版者・発行元
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Mutations in keratoepithelin are associated with blinding ocular diseases, including lattice corneal dystrophy type 1 and granular corneal dystrophy type 2. These diseases are characterized by deposits of amyloid fibrils and/or granular non-amyloid aggregates in the cornea. Removing the deposits in the cornea is important for treatment. Previously, we reported the destruction of amyloid fibrils of beta(2)-microglobulin K3 fragments and amyloid beta by laser irradiation coupled with the binding of an amyloid-specific thioflavin T. Here, we studied the effects of this combination on the amyloid fibrils of two 22-residue fragments of keratoepithelin. The direct observation of individual amyloid fibrils was performed in real time using total internal reflection fluorescence microscopy. Both types of amyloid fibrils were broken up by the laser irradiation, dependent on the laser power. The results suggest the laser-induced destruction of amyloid fibrils to be a useful strategy for the treatment of these corneal dystrophies.
- リンク情報
- ID情報
-
- DOI : 10.1074/jbc.M111.222901
- ISSN : 0021-9258
- Web of Science ID : WOS:000288547000099