2011年5月
Expressing exogenous genes in newts by transgenesis
NATURE PROTOCOLS
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 6
- 号
- 5
- 開始ページ
- 600
- 終了ページ
- 608
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/nprot.2011.334
- 出版者・発行元
- NATURE PUBLISHING GROUP
The great regenerative abilities of newts provide the impetus for studies at the molecular level. However, efficient methods for gene regulation have historically been quite limited. Here we describe a protocol for transgenically expressing exogenous genes in the newt Cynops pyrrhogaster. This method is simple: a reaction mixture of I-SceI meganuclease and a plasmid DNA carrying a transgene cassette flanked by the enzyme recognition sites is directly injected into fertilized eggs. The protocol achieves a high efficiency of transgenesis, comparable to protocols used in other animal systems, and it provides a practical number of transgenic newts (similar to 20% of injected embryos) that survive beyond metamorphosis and that can be applied to regenerative studies. The entire protocol for obtaining transgenic adult newts takes 45 months.
- リンク情報
- ID情報
-
- DOI : 10.1038/nprot.2011.334
- ISSN : 1754-2189
- Web of Science ID : WOS:000290273300005