KITOH Katsuya

© 2015. Tachyzoites of Toxoplasma gondii, an obligate intracellular parasite, actively invade a broad spectrum of cell types. T. gondii infects leukocytes and spreads to distant organs such as the brain, lungs and muscles. However, the mechanism o...

© 2015 Elsevier Ireland Ltd. We investigated the presence of antibodies against Toxoplasma gondii in domesticated goats intended for human consumption in a rural suburb of Rajshahi, Bangladesh. Antibodies to T. gondii were found in 55.1% (80/145) ...

© 2015 Elsevier Inc. Tachyzoites of Toxoplasma gondii, an obligate intracellular parasite, actively invade almost all types of nucleated cells. However, T. gondii tachyzoites preferentially infect particular types of animal tissue cells. The mecha...

The presence of anti-erythrocyte autoantibodies in animals infected with various Babesia species is well reported. However, the pathogenesis of autoantibodies in babesiosis is poorly understood. Here, we demonstrated that anti-erythrocyte immunogl...

Toxoplasma gondii is an obligate intracellular apicomplexan parasite that infects humans and animals. Ingested parasites cross the intestinal epithelium, invade leukocytes and are then disseminated to peripheral organs. However, the mechanism of e...

© 2014 The Japanese Society of Veterinary Science. Toxoplasma gondii is a zoonotic protozoan parasite that infects humans and domestic animals. In this study, the seroprevalence of T. gondii antibodies was investigated using serum samples collecte...

Bovine abortion caused by the Apicomplexan parasite Neospora caninum is a major economic problem in the livestock industry worldwide. Our study measured the prevalence and temporal changes in levels of antibodies specific for two N. caninum derive...

The intracellular parasite Toxoplasma gondii is thought to disseminate throughout the host by circulation of tachyzoite-infected leukocytes in the blood, and adherence and migration of such leukocytes into solid tissues. However, it is unclear whe...

Hemolytic activity for animals infected with various Babesia species is well reported. In this study, we confirmed that serum of Babesia rodhaini-infected mice also showed hemolytic activity. Erythrocytes from non-infected mice were lysed by co-in...

The seroprevalence of Toxoplasma gondii was examined in adult women and domestic animals used for meat products from the Mymensingh District, Bangladesh. Cattle, goats and sheep showed a high seroprevalence (12, 32 and 40%, respectively), while th...

In mammals with a hemochorial placenta (e.g., primates and rodents), the maternal and fetal bloodstreams are separated by the blood-placenta barrier. However, a few maternal cells in the general circulation pass through the barrier during normal p...

The apicomplexan, obligate intracellular parasite Toxoplasma gondii orally infects humans and animals. The parasites cross the intestinal epithelium, invade leukocytes in the general circulation and then disseminate into the peripheral organs. The...

A case of multiple primary tumors observed in the heart base and in the lung of a 7-year-old intact female, flat-coated retriever was reported. Morphological differences between both tumors and detailed immunohistochemical study revealed that the ...

Babesia gibsoni (B. gibsoni) is a tick-borne hemoprotozoan parasite, which causes piroplasmosis in dogs. Diagnosis of canine babesiosis is commonly carried out using Giemsa-stained thin blood smears. However, at low levels of infection, it is diff...

Toxoplasma gondii is an intracellular parasite. It has been thought that T. gondii can disseminate throughout the body by circulation of tachyzoite-infected leukocytes (intracellular parasite) in the blood flow. However, a small number of parasite...

Detection of the initial site of Toxoplasma gondii reactivation in brain tissue is difficult because the number of latent cysts is small and reactivation is a transient event. To detect the early stage of reactivation in mouse brain tissue, we con...

A 4-months-old calf of Japanese black cattle was diagnosed with orotic aciduria by gas-chromatography/mass-spectromerty (GC/MS). Until now orotic aciduria had not been reported in Japanese black cattle. The animal showed repeated diarrhea. The hem...

OBJECTIVES: To retrospectively compare the data taken at the first visit of 34 cats with chronic renal failure surviving more than one month (surviving group) and 16 cats dying within one month (non-surviving group). METHODS: Records were collecte...

Objective - To investigate effects of short- and long-term administration of glucocorticoids, feeding status, and serum concentrations of insulin and cortisol on plasma leptin concentrations in dogs. Animals - 20 nonobese dogs. Procedure - For exp...

Recently we reported that a chimeric molecule containing mouse transferrin receptor and immunoglobulin G1 (IgG1) Fc, mTR-Fc, induced higher immune responses and can be used as a vaccine adjuvant. In this study, the immunological property of the mo...

Endocrine patterns were compared in 2 strains of Japanese black cattle with growth retardation; MHO- and HSK-paternal strains (MHO and HSK cattle, respectively). MHO cattle (n=8) displayed lower serum concentrations of insulin-like growth factor-1...

In order to determine insulin secretability and glucose utilization, a glucose tolerance test was performed in ateliotic cattle of 2 paternal strains; MHO and HSK cattle. MHO and HSK cattle showed different endocrine patterns in our previous study...

To elucidate the cause of growth retardation, 14 cattle with renal tubular dysplasia (RTD) were investigated by determining serum growth hormone (GH) and insulin-like growth factor (IGF)-1 concentrations. Compared with 6 healthy cattle, the baseli...

Between September, 2001, and March, 2002, the presence of intestinal parasites was investigated from fecal conditions of 1, 535 puppies brought from 214 breeding kennels to determine their state of health before their being shipped to pet shops. T...

To examine whether an angiotensin converting enzyme (ACE) inhibitor, benazepril, can be transformed to the active metabolite, benazeprilat, by severely injured liver of dogs with ascitic heartworm disease, benazepril hydrochloride was administered...

To detect metabolic abnormalities, we performed gas chromatography/mass spectrometry (GC/MS) on growth-retarded Japanese Black cattle. Many organic acids, amino acids, sugars, nucleic-acid bases, and glycine conjugates were detected in urine from ...

Detection ability, sensitivity, and specificity of a new test kit (New Kit) for detecting circulating adult heartworm antigens by means of the immunochromatography method were compared with those of an enzyme immunoassay test kit (Control A) and a...

Prothrombin time (PT), activated partial thromboplastin time (APTT), and plasma fibrinogen concentration (pfg) were measured to evaluate the usefulness of a dry-system coagulation analyzer (DRY method) to detect canine blood coagulopathy. The samp...

Examination of their clinical significance showed that plasma leptin concentrations were significantly higher in 15 obese cats than in normal controls (P<0.01) and correlated significantly with body weight, chest and abdominal measurements, relati...

The angiotensin-converting enzyme inhibitor, benazepril was orally administered to dogs for a period 15days before and after renal ablation. Renal ablation by right-side nephrectomy and ligation of one branch of the left renal artery were performe...

Effect of adult heartworm (HW) crude extract on isolated canine abdominal aortic strips precontracted with noradrenaline was examined by recording isometric changes in tension. HW extract caused contraction of the aortic strip at a low concentrati...

To determine whether heartworm (HW) extract-induced shock in dogs is consistent with anaphylactic shock by examining the role of histamine. Animals-6 mixed-breed dogs (3 without and 3 with HW infections) and 4 specific pathogen-free (SPF) Beagles....

Objective - To compare the mechanisms of heartworm (HW) extract-induced shock and endotoxin-induced shock in dogs by determination of serum tumor necrosis factor (TNF) concentrations. Animals - 11 mixed-breed dogs (7 without and 4 with HW infectio...

To elucidate one way of the shock mechanisms, the hemodynamic alterations were examined in 7 dogs with heartworm (HW) extract-induced shock. The first alteration observed after injection of HW extract was a decrease in right ventricular end-diasto...

In order to confirm the effects of matching of expressed feline major histocompatibility complex (FLA) class II DRB genotype on transplantation immunity in cats, skin-allogeneic transplantation was carried out between cats, in which DRB genes expr...

Clinical findings and thyroid function were examined in8 growth-retarded Japanese Black cattle. Until about 3 or4 months of age, the animals had grown normally and had had normal appetites. At initial examination (3 to 13 months of age), however, ...

The clinical significance of plasma leptin concentration was examined in 45 obese dogs. Significantly higher than those in 28 control dogs, the obese dogs' plasma leptin concentrationswere correlated closely with body weight; chest size; abdominal...

Examination of a male, mixed-breed dog (12 years old) with severe anemia, bleeding tendency, increased serum indirect bilirubin concentration, acanthocytosis of the peripheral blood, and severe hemoglobinuria revealed thrombocytopenia, prolonged b...

Various hereditary diseases analogous to particular hman heritable diseases have been identified in cattle. Investigation of these cattle diseases will provide useful information regarding the pathogenesis of the corresponding human diseases. Rena...

Renal tubular dysplasia is a hereditary disease of Japanese black cattle showing renal failure and growth retardation with an autosomal recessive trait. In the present study, we mapped the locus responsible for the disease (RTD) by linkage analysi...

The optimal condition for methods of lymphocytotoxic crossmatch test for feline renal transplantation was investigated. On separation of viable lymphocytes from whole blood, the best results were obtained when Ficoll-diatrizoate with 1.078 of a sp...

Objective - To examine pathologic findings in dogs with shock induced by IV administration of heartworm (HW) extract. Animals - 22 mixed-breed adult dogs. Procedures - Heartworm extract was administered IV, and pathologic changes in dogs that died...

The reliability of a newly devised serodiagnosis test kit aiming at more simple procedure with smaller sample volumes for detection of circulating adult heartworm antigen (enzyme immunoassay), was compared with that of a conventional kit. Forty-on...

Effect of milbemycin oxime (MO) by oral route was evaluated in dogs with spontaneous demodicosis and mange. Three dogs with demodicosis given MO at 0.5 to 1 mg/kg did not show any improvement of the dermal signs, and the number of demodex mites in...

Eighteen puppies naturally infected with Toxocara canis received orally 0.25 mg/kg milbemycin oxime (MO). In 12 dogs, eggs per gram feces (EPG) became negative by 30 days after treatment when no adult worms were recovered. In 4 dogs, the fecal EPG...

A crude, whole-body extract of female or male heartworms was injected IV into 28 dogs with and 22 dogs without heartworm (HW) infection. The female HW extract caused shock in 22 of 24 dogs with and 12 of 20 dogs without HW infection. The male HW e...

A crude, whole-body extract of female heartworms was administered IV to 10 dogs with and 13 dogs without heartworm (HW) infection. Shock developed in 8 of 10 infected dogs and 11 of 13 non-infected dogs, and blood coagulopathy was observed in 12 o...

The accuracy of a latex agglutination (LA) test kit for detection of circulating adult heartworm antigen was compared with that of a enzyme immunoassay (ELISA) test kit. Thirty-five (83%) and 41 (98%) of 42 heartworm-free dogs were negative by LA ...

The efficacy of a low-mineral diet (Felistar low mineral) on dissolution and prevention of urine struvite crystals was evaluated in 15 cats. After feeding a wet-type food for dissolution, struvite crystals disappeared with decreased urine pH in 11...

To recognize the stage conversion of Toxoplasma gondii between tachyzoite and bradyzoite in live host cells, a transgenic T. gondii line, which expressed stage-specific red and green fluorescence, was constructed. T. gondii PLK strain tachyzoites ...

To investigate systemic oxygen (O2) transport, we calculated the oxygen delivery index (Do2I), oxygen consumption index (Vo2I) and oxygen extraction ratio (ER) in dogs with heartworm (HW) disease. The Do2I was 770 +/- 331 ml/min/kg in dogs mildly ...

Serum concentrations of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) were determined in 5 calves in the same lineage with growth retardation. They had normal appetites, activities, body proportion, and laboratory test results. Calv...

The concentrations of magnesium and calcium in the serum and urine and their rates of clearance were determined in cattle with renal tubular dysplasia, an autosomal recessive hereditary disease associated with a deletion of the paracellin-1 gene i...

Relationships among plasma renin activities (PRA), plasma angiotensin II (ATII) concentrations, atrial natriuretic peptide (ANP) concentrations and cardiopulmonary function values were examined in dogs with ascitic pulmonary heartworm disease and ...

In order to examine the safety of an angiotensin-converting enzyme (ACE) inhibitor in dogs with impaired renal excretion route, benazepril was administered orally, and plasma concentrations of benazeprilat, the active metabolite of benazepril, wer...

Pathological studies were conducted on 91 Japanese Black cattle with a hereditary disease which induced growth retardation, long hooves and renal failure. In calves one to two months old, no gross abnormalities were observed in the kidneys, but mi...

Four of 51 dogs with chronic dermatitis were made to react to crude Japanese cedar (Cryptomeria japonica, CJ) pollen allergen by the intradermal skin test (IDST). These four dogs had specific IgE to CJ as determined by Prausnitz-Küstner test. In t...

For genotypitig of feline major histocompalibility complex (FLA) class II DRB, the polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method using group-specific primers was tried. Sixty-six DRB genes were classified i...

Benazepril (BP), an angiotensin convertive enzyme inhibitor, was administered orally once daily for 4 weeks to 31 dogs with mild to moderate (NYHA functional classes II and III) congestive heart failure caused from mitral insufficiency (MI). There...

Objective - To compare results of laboratory tests in dogs with vena caval syndrome before and after surgical removal of heartworms. Design - Longitudinal uncontrolled clinical trial. Animals - 51 dogs with vena caval syndrome. Procedure - Heartwo...

Laboratory data in 47 dogs with caval syndrome (CS) surviving after surgical HW removal compared with those in 15 dogs with CS which died after the treatment. The number of HWs removed in surviving dogs was significantly greater than in nonsurvivi...

A new hereditary disease characterised by renal failure, poor growth and long hooves in Japanese Black cattle (wagyu) has been recognised in a region of central Japan since 1990. The number of calves affected has increased gradually, with the inci...

Blood gases and cardiopulmonary function were analyzed in 67 dogs with heartworm (HW) caval syndrome (CS) and 19 HW-free dogs. Arterial oxygen tension (PaO2) was 91.5 +/- 7.3 mmHg in the HW-free dogs, 74.9 +/- 14.3 mmHg in 46 dogs that subsequentl...

Clinical
evaluated in 59 Collies consisting of 16 rough-coated Collies, 40 Shelties, and 3 Bearded Collies. Milbemycin oxime was administered orally to the dogs 6 times from June to November at a monthly dose of over 0.25 mg/kg (prophylactic dose ...

The Simplate is a device developed for bleeding time determination in man. It was applied to dogs with efficiency. In dogs, bleeding time determination was performed on the dorsum auricularis. In it, pressure on the ear was maintained constant by ...