2001年5月
Impairment of Gingival Fibroblast Adherence by IL-6/sIL-6R
Journal of Dental Research
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- 巻
- 80
- 号
- 5
- 開始ページ
- 1421
- 終了ページ
- 1424
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1177/00220345010800050701
- 出版者・発行元
- SAGE Publications
Interleukin-6 (IL-6) binds to human gingival fibroblasts (HGF) in the presence of a soluble form of IL-6 receptor (sIL-6R). We investigated the effects of IL-6 on the functions of HGF in the presence of sIL-6R. HGF changed their morphology from spindle-shaped to round, and detached from the culture dish by stimulation with IL-6/sIL-6R. In this condition, a signal transducer gpl30 and a transcription factor Stat3 were phosphorylated, resulting in activation of transcription factors Stat3 and C/EBPβ. Cytoskeletal β-actin and adhesion molecule integrin-a5, a subunit of α5β1 integrin (VLA-5), were found to possess potential binding domains for these transcription factors in their promoters. Accumulation of (3-actin and integrin-a5 mRNA decreased, contrary to the expectation of the induction of gene transcription. Furthermore, the decrease in their mRNAs was associated with reduced expression of both actin and VLA-5 proteins. These results suggest that the expression of VLA-5 and actin was down-regulated in HGF through an IL-6 signaling pathway, resulting in impairment of HGF adherence.
- リンク情報
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- DOI
- https://doi.org/10.1177/00220345010800050701
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11437212
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000171026800007&DestApp=WOS_CPL
- URL
- http://journals.sagepub.com/doi/pdf/10.1177/00220345010800050701
- ID情報
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- DOI : 10.1177/00220345010800050701
- ISSN : 0022-0345
- eISSN : 1544-0591
- ORCIDのPut Code : 47542638
- PubMed ID : 11437212
- Web of Science ID : WOS:000171026800007