2017年
Generation and analysis of cartilage-specific CCN2 overexpression in transgenic mice
Methods in Molecular Biology
- ,
- ,
- 巻
- 1489
- 号
- 開始ページ
- 391
- 終了ページ
- 403
- 記述言語
- 英語
- 掲載種別
- 論文集(書籍)内論文
- DOI
- 10.1007/978-1-4939-6430-7_32
- 出版者・発行元
- Humana Press Inc.
Recent progress in gene-editing technology has provided a strong impact for improved our understanding of molecular functions in living organisms. Here we describe our method to generate transgene-overexpressing mouse models, which method involves the use of tissue-specific promoters for analyzing a certain molecule (s) in special tissues. The protocol described in this chapter uses the Col2a1 promoter-enhancer, which is known for driving specific and strong transgene expression in cartilage and is based on several of our studies showing a positive role of the connective tissue growth factor (CCN2) in cartilage-bone development and maintenance of articular cartilage. These mice show strongly accelerated endochondral ossification resulting in enhanced bone elongation, as well as resistance to age-related articular degeneration. This protocol also describes how to analyze the molecular mechanisms of these phenomena by use of chondrocytes isolated from CCN2-overexpressing cartilage.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-4939-6430-7_32
- ISSN : 1064-3745
- PubMed ID : 27734391
- SCOPUS ID : 84991669748