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Mar, 2017

Measurement of pyridoxal 5 '-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children

CLINICA CHIMICA ACTA
  • Tomoyuki Akiyama
  • Mari Akiyama
  • Yumiko Hayashi
  • Takashi Shibata
  • Yoshiyuki Hanaoka
  • Soichiro Toda
  • Katsumi Imai
  • Shin-ichiro Hamano
  • Tohru Okanishi
  • Harumi Yoshinaga
  • Katsuhiro Kobayashi
  • Display all

Volume
466
Number
First page
1
Last page
5
Language
English
Publishing type
DOI
10.1016/j.cca.2016.12.027
Publisher
ELSEVIER SCIENCE BV

Background: We quantified pyridoxal 5'-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers.
Methods: CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PI, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models.
Results: The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated.
Conclusions: We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy. (C) 2016 Elsevier B.V. All rights reserved.

Link information
DOI
https://doi.org/10.1016/j.cca.2016.12.027
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000397834600001&DestApp=WOS_CPL
ID information
  • DOI : 10.1016/j.cca.2016.12.027
  • ISSN : 0009-8981
  • eISSN : 1873-3492
  • Web of Science ID : WOS:000397834600001

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