Hisatake Koji

J-GLOBAL         Last updated: Jan 16, 2020 at 02:49
Hisatake Koji
University of Tsukuba
Faculty of Medicine
Job title

Research Areas


Published Papers

Fukuda, Aya; Honda, Shiho; Fujioka, Norie; Sekiguchi, Yuya; Mizuno, Seiya; Miwa, Yoshihiro; Sugiyama, Fumihiro; Hayashi, Yohei; Nishimura, Ken; Hisatake, Koji
PloS one   14(11)    Apr 2019
Uncoupling protein 1 (UCP1) is a mitochondrial protein that is expressed in both brown and beige adipocytes. UCP1 uncouples the mitochondrial electron transport chain from ATP synthesis to produce heat via non-shivering thermogenesis. Due to their...
Bui, Phuong Linh; Nishimura, Ken; Seminario Mondejar, Gonzalo; Kumar, Arun; Aizawa, Shiho; Murano, Kensaku; Nagata, Kyosuke; Hayashi, Yohei; Fukuda, Aya; Onuma, Yasuko; Ito, Yuzuru; Nakanishi, Mahito; Hisatake, Koji
Cell reports   29(7) 1909-1922.e5   Nov 2019
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) is accompanied by dramatic changes in epigenetic programs, including silencing of endogenous and exogenous retroviruses. Here, we utilized replication-defective and persistent S...
Nishimura, Ken; Fukuda, Aya; Hisatake, Koji
International Journal of Molecular Sciences   20(9)    May 2019   [Refereed]
Pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), hold a huge promise for regenerative medicine, drug development, and disease modeling. PSCs have unique metabolic features that are ak...
Nishimura, Ken;Ishiwata, Hiroshi;Sakuragi, Yuta;Hayashi, Yohei;Fukuda, Aya;Hisatake, Koji
Scientific Reports   9 1777   Feb 2019   [Refereed]
Pluripotent stem cells (PSCs) have various degrees of pluripotency, which necessitates selection of PSCs with high pluripotency before their application to regenerative medicine. However, the quality control processes for PSCs are costly and time-...
Tran, Thi Hai Yen; Fukuda, Aya; Aizawa, Shiho; Bui, Phuong Linh; Hayashi, Yohei; Nishimura, Ken; Hisatake, Koji
Biochemistry and biophysics reports   15 86-92   Sep 2018   [Refereed]
Generation of induced pluripotent stem cells (iPSCs) with naive pluripotency is important for their applications in regenerative medicine. In female iPSCs, acquisition of naive pluripotency is coupled to X chromosome reactivation (XCR) during soma...
Promotion of iPSC generation through a metabolic shift by Tcl1
西村, 健;久武幸司
医学のあゆみ   265(4) 293-294   Apr 2018
西村, 健;Tran, Vo Kieu Nhi;久武幸司
PHARMASTAGE   17(10) 11-15   Jan 2018
Nishimura, Ken; Ohtaka, Manami; Takada, Hitomi; Kurisaki, Akira; Tran, Nhi Vo Kieu; Tran, Yen Thi Hai; Hisatake, Koji; Sano, Masayuki; Nakanishi, Mahito
Stem cell research   23 13-19   Jun 2017   [Refereed]
Transgene-free induced pluripotent stem cells (iPSCs) are valuable for both basic research and potential clinical applications. We previously reported that a replication-defective and persistent Sendai virus (SeVdp) vector harboring four reprogram...
Stem Cell Reports   3(5) 915-929   Nov 2014   [Refereed]
The detailed mechanism of reprogramming somatic cells into induced pluripotent stem cells (iPSCs) remains largely unknown. Partially reprogrammed iPSCs are informative and useful for understanding the mechanism of reprogramming but remain technica...
Fukuda, Aya; Shimada, Miho; Nakadai, Tomoyoshi; Nishimura, Ken; Hisatake, Koji
PLOS ONE   8(8)    Aug 2013   [Refereed]
DSIF, the Paf1 complex, and Tat-SF1 have nonredundant, cooperative roles in RNA polymerase II elongation
Genes & Development   23(23) 2765-2777   Dec 2009   [Refereed]
Transcription elongation factor DSIF/Spt4-Spt5 is capable of promoting and inhibiting RNA polymerase II elongation and is involved in the expression of various genes. While it has been known for many years that DSIF inhibits elongation in collabor...
Immunoelectron microscopy of adult T cell leukemia-associated antigen by the peroxidase-labeled antibody method.
Oda, T;Watanabe, S;Araki, Y;Hatsushika, M;Sumi, H;Nakamura, T;Suma, F;Hisatake, K
Cellular and Molecular Biology   31(1) 49-56   Jan 1985   [Refereed]
Sequence-specific binding of a transcription factor TFID to the promoter region of mouse ribosomal RNA gene.
Tanaka, N;Kato, H;Ishikawa, Y;Hisatake, K;Tashiro, K;Kominami, R;Muramatsu, M
The Journal of Biological Chemistry   256(23) 12836-13842   Aug 1990   [Refereed]
Structure of the core promoter of human and mouse ribosomal RNA gene: Asymmetry of species-specific transcription.
Ishikawa, Y;Safrany, G;Hisatake, K;Tanaka, N;Maeda, Y;Kato, H;Kominami, R;Muramatsu, M
Journal of Molecular Biology   218(1) 55-67   Mar 1991   [Refereed]
Cloning and structural analysis of cDNA and its gene for mouse transcription factor UBF.
Hisatake, K;Nishimura, T;Maeda, Y;Hanada, K;Song, C;Muramatsu, M
Nucleic Acids Research   19(17) 4631 4637   Sep 1991   [Refereed]
Sequence of general transcription factor TFIIB and relationships to other initiation factors.
Malik, S;Hisatake, K;Sumimoto, H;Horikoshi, M;Roeder, RG
Proceedings of the National Academy of Sciences of the United States of America   88(21) 9553-9557   Nov 1991   [Refereed]
Conserved structural motifs between Xenopus and human TFIIB.
Hisatake, K;Malik, S;Roeder, RG;Horikoshi, M
Nucleic Acids Research   19(23) 6639   Dec 1991   [Refereed]
Isolation and characterization of a cDNA encoding Drosophila transcription factor TFIIB.
Yamashita, S;Wada, K;Horikoshi, M;Gong, D-W;Kokubo, T;Hisatake, K;Yokotani, N;Malik, S;Roeder, RG;Nakatani, Y
Proceedings of the National Academy of Sciences of the United States of America   89(7) 2839-2843   Apr 1992   [Refereed]
Mouse rRNA transcription factor mUBF requires both HMG-box1 and an acidic tail for nucleolar accumulation: molecular analysis of the nucleolar targeting mechanism.
Maeda, Y;Hisatake, K;Kondo, T;Hanada, K;Song, C;Nishimura, T;Muramatsu, M
The EMBO Journal   11(10) 3695-3704   Oct 1992   [Refereed]
The p250 subunit of native TATA box-binding factor TFIID is the cell cycle regulatory protein CCG1.
Hisatake, K;Hasegawa, S;Takada, R;Nakatani, Y;Horikoshi, M;Roeder, RG
Nature   362(6416) 179-181   Mar 1993   [Refereed]
Functional dissection of TFIIB domains required for TFIIB-TFIID-promoter complex formation and basal transcription activity.
Hisatake, K;Roeder, RG;Horikoshi, M
Nature   363(6431) 744-747   Jun 1993   [Refereed]
Transcription factor TFIIB sites important for interaction with promoter-bound TFIID.
Yamashita, S;Hisatake, K;Kokubo, T;Doi, K;Roeder, RG;Horikoshi, M;Nakatani, Y
Science   261(5120) 463-467   Jul 1993   [Refereed]
Regulation of mouse UBF gene by multiple growth-related control elements.
Nishimura, T;Hanada, K;Maeda, Y;Song, C;Hisatake, K;Muramatsu, M
Biochemical and Biophysical Research Communications   205(2) 1217-1225   Dec 1994   [Refereed]
Identification of an enhancer responsible for a tumor marker gene expression by means of transgenic rats.
Suzuki, T;Imagawa, M;Hirabayashi, M;Yuki, A;Hisatake, K;Muramatsu, M
Cancer Research   55(12) 2651-2655   Jun 1995   [Refereed]
Evolutionary conservation of human TATA-binding-polypeptide associated factors TAFII31 and TAFII80 and interactions of TAFII80 with other TAFs and with general transcription factors.
Hisatake, K;Ohta, T;Takada, R;Guermah, M;Horikoshi, M;Nakatani, Y;Roeder, RG
Proceedings of the National Academy of Sciences of the United States of America   92(18) 8195-8199   Aug 1995   [Refereed]
Crystal structure of a TFIIB-TBP-TATA-element ternary complex.
Nikolov, DB;Chen, H;Halay, ED;Usheva, AA;Hisatake, K;Lee, DK;Roeder, RG;Burley, SK
Nature   377(6545) 119-128   Sep 1995   [Refereed]
Unliganded thyroid hormone receptor a can target the TATA-binding protein for transcriptional repression.
Fondell, GD;Brunel, F;Hisatake, K;Roeder, RG
Molecular and Cellular Biology   16(1) 281-287   Jan 1996   [Refereed]
The fission yeast rpa17+ gene encodes a functional homolog of AC19, a subunit of RNA polymerase I and III of Saccharomyces cerevisiae.
Imai, K;Imazawa, Y;Yao, Y;Yamamoto, K;Hisatake, K;Muramatsu, M;Nogi, Y
Molecular and General Genetics   261(2) 364-373   Mar 1999   [Refereed]
Isolation and characterization of the fission yeast gene rpa42+m which encodes a subunit shared by RNA polymerae I and III.
Imazawa, Y;Imai, K;Fukushima, A;Hisatake, K;Muramatsu, M;Nogi, Y
Molecular and General Genetics   262(4-5) 749-757   Dec 1999   [Refereed]
The Rpb6 subunit of fission yeast RNA polymerase II is a contact target of the transcription elongation factor TFIIS.
Ishiguro, A;Nogi, Y;Hisatake, K;Muramatsu, M;Ishihama, A
Molecular and Cellular Biology   20(4) 1263-1270   Feb 2000   [Refereed]
FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between p-TEFb and TFIIH.
Wada, T;Orphanides, G;Hasegawa, J;Kim, D-K;Shima, D;Yamaguchi, Y;Fukuda, A;Hisatake, K;Oh, S;Reinberg, D;Handa, H
Molecular Cell   5(6) 1067-1072   Jun 2000   [Refereed]
Isolation and characterization of the fission yeast gene Sprpa12+ reveals that the conserved C-terminal zinc-finger region is dispensable for the function of its product.
Imazawa, Y;Imai, Y;Yao, Y;Yamamoto, K;Hisatake, K;Muramatsu, M;Nogi, Y
Molecular and General Genetics   264(6) 852-859   Feb 2001   [Refereed]
Reconstitution of recombinant TFIIH that can mediate activator-dependent transcription.
Fukuda, A;Yamauchi, J;Wu, SY;Chiang, CM;Muramatsu, M;Hisatake, K
Genes to Cells   6(8) 707-719   Aug 2001   [Refereed]
Regulation of receptor activator of NF-kB ligand-induced tartrate-resistant acid phosphatase gene expression by PU.1-interacting protein/interferon regulatory factor-4 synergism with microophthalmia transcription factor.
Matsumoto, M;Hisatake, K;Nogi, Y;Tsujimoto, M
The Journal of Biological Chemistry   276(35) 33086-33092   Aug 2001   [Refereed]
The regulatory role for the ERCC3 helicase of general transcription factor TFIIH during promoter escape in transcriptional activation.
Fukuda, A;Nogi, Y;Hisatake, K
Proceedings of the National Academy of Sciences of the United States of America   99(3) 1206-1211   Feb 2002   [Refereed]
The fission yeast RPA21 subunit of RNA polymerase I: an evolutionarily conserved subunit interacting with ribosomal DNA (rDNA) transcription factor Rrn3p for recruitment to rDNA promoter.
Imazawa, Y;Hisatake, K;Nakagawa, K;Muramatsu, M;Nogi, Y
Genes and Genetic System   77(3) 147-157   Jun 2002   [Refereed]
Alleviation of PC4-mediated transcriptional repression by the ERCC3 helicase activity of general transcription factor TFIIH.
Fukuda, A;Tokonabe, S;Hamada, M;Matsumoto, M;Tsukui, T;Nogi, Y;Hisatake, K
The Journal of Biological Chemistry   278(17) 14827-14831   Apr 2003   [Refereed]
The fission yeast RPA51 is a functional homolog of the budding yeast A49 subunit of RNA polymerase I and required for maximizing transcription of ribosomal DNA.
Nakagawa, K;Hisatake, K;Imazawa, Y;Ishiguro, A;Matsumoto, M;Pape, L;Ishihama, A;Nogi, Y
Genes and Genetic System   78(3) 199-209   Jun 2003   [Refereed]
Transcriptional coactivator PC4 stimulates promoter escape and facilitates transcriptional synergy by GAL4-VP16.
Fukuda, A;Nakadai, T;Shimada, M;Tsukui, T;Matsumoto, M;Nogi, Y;Meisterernst, M;Hisatake, K
Molecular and Cellular Biology   24(14) 6525-6535   Jul 2004   [Refereed]
Essential role of p38 MAP kinase in Cathepsin K gene expression during osteoclastogenesis through association of NFATc and PU.1.
Matsumoto, M;Kogawa, M;Wada, S;Takayanagi, H;Tsujimoto, M;Katayama, S;Hisatake, K;Nogi, Y
The Journal of Biological Chemistry   279(44) 45969-45979   Oct 2004   [Refereed]
ABT1-associated protein (ABTAP), a novel nuclear protein conserved from yeast to mammals, represses transcriptional activation ABT1.
Oda, T;Fukuda, A;Hagiwara, H;Masuho, Y;Muramatsu, M;Hisatake, K;Yamashita, T
Journal of Cellular Biochemistry   93(4) 788-806   Nov 2004   [Refereed]
The fission yeast protein, Ker1p, is an ortholog of RNA polymerase I subunit A14 in Saccharomyces cerevisiae, and is required for stable association of Rrn3p and RPA21 in Pol I.
Imazawa, Y;Hisatake, K;Mitsuzawa, H;Matsumoto, M;Tsukui, T;Nakagawa, K;Nakadai, T;Shimada, M;Ishihama, A;Nogi, Y
The Journal of Biological Chemistry   280(12) 11467-11474   Mar 2005   [Refereed]
基本転写因子とその関連疾患 (特集 転写制御と疾患--遺伝子のon-offと病態)
久武, 幸司
Molecular medicine   42(9) 973-981   Sep 2005
Histone methylation and transcriptional regulation
生化学   79(4) 362-365   Apr 2007
Yamagata, Kazuyuki;Daitoku, Hiroaki;Takahashi, Yuta;Namiki, Kana;Hisatake, Koji;Kako, Koichiro;Mukai, Hidehito;Kasuya, Yoshitoshi;Fukamizu, Akiyoshi
MOLECULAR CELL   32(2) 221-231   Oct 2008   [Refereed]
Fukuda, Aya;Nakadai, Tomoyoshi;Shimada, Miho;Hisatake, Koji
JOURNAL OF BIOLOGICAL CHEMISTRY   284(35) 23472-23480   Aug 2009   [Refereed]
Shimada, Miho;Nakadai, Tomoyoshi;Fukuda, Aya;Hisatake, Koji
JOURNAL OF BIOLOGICAL CHEMISTRY   285(13) 9390-9401   Mar 2010   [Refereed]

Books etc

久武,幸司 (Part:Contributor, 蛋白質解析法)
Feb 2012   
Histone methylation and transcriptional regulation
Aya Fukuda; Koji Hisatake; (Part:Supervisor)
biochemistry The Japanese Biochemical Society   Apr 2007   
Mechanism of histone H3 phosphorylation upon induction of c-fos gene transcription
Miho Shimada; Tomoyoshi Nakadai; Aya Fukuda; Koji Hisatake; (Part:Supervisor)
Jikken Igaku, vol.25, No.10 Yodosha   Jun 2007   

Conference Activities & Talks

CARS 分光イメージングによる生体組織の ex vivo 観察 [Invited]
加納, 英明;湯口周;福田綾;久武幸司;加野准子;野口雅之
第16回医用分光学研究会   21 Nov 2018   
後方散乱配置による生体組織のex vivoマルチモーダル非線形光学イメージング
Kanou, Hideaki;湯口周;福田綾;久武幸司;野口雅之
第12回分子科学討論会   10 Sep 2018   
非線形光学イメージングを用いた iPS 細胞のリプログラミング過程の研究
加納, 英明;丸山拓史;米山弘亮;西村健;久武幸司
平成30年度 日本分光学会年次講演会   22 May 2018   
加納, 英明;武井祐樹;平井理愛;下平雄貴;島田林太郎;福田綾;久武幸司
平成30年度 日本分光学会年次講演会   22 May 2018   
Analysis on transcriptional regulation in the early phase of somatic cell reprogramming
FUKUDA, AYA;Anh, Le Phuong Hoang;Tetsuo, Kato;TL, Nguyen;Ken, Nishimura;Koji, Hisatake
Tsukuba Global Science Week 2018   Sep 2018   


特願2017-223802 : 遺伝子改変非ヒト動物及びその使用