MISC

1999年4月

Myosin light chain kinase inhibitors and calmodulin antagonists inhibit Ca2+- and ATP-dependent catecholamine secretion from bovine adrenal chromaffin cells

JOURNAL OF AUTONOMIC PHARMACOLOGY
  • C Matsumura
  • ,
  • H Kuwashima
  • ,
  • T Kimura

19
2
開始ページ
115
終了ページ
121
記述言語
英語
掲載種別
DOI
10.1046/j.1365-2680.1999.00125.x
出版者・発行元
WILEY-BLACKWELL

1 We have used stage-specific assays for ATP-dependent priming and for Ca2+-activated triggering in the absence of ATP to examine the effects of myosin light chain kinase (MLCK) inhibitors, ML-9 and ML-7, and calmodulin antagonists, W-7 and trifluoperazine (TFP), on regulated exocytosis in beta-escin-permeabilized bovine adrenal chromaffin cells.
2 Ca2+ (0.1-30 mu M) induced a significantly greater secretion of catecholamines in the presence of MgATP (2 mM) than in the absence of MgATP.
3 ML-9 (30 and 100 mu M), ML-7 (30 and 100 mu M), W-7 (30 and 100 I-LM) and TFP (10 and 30 mu M) inhibited the Ca2+-induced catecholamine secretion in the presence of MgATP, but did not affect the catecholamine response to Ca2+ in the absence of MgATP.
4 In intact cells all these compounds inhibited catecholamine secretion in responses to acetylcholine (100 mu M) and high K+ (40 mM).
5 The results obtained in permeabilized cells suggest that the calmodulin-MLCK system plays an essential role in the ATP-requiring priming stage but not in the Ca2+-triggered fusion step in the exocytotic process in bovine adrenal chromaffin cells.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1046/j.1365-2680.1999.00125.x
CiNii Articles
http://ci.nii.ac.jp/naid/10010527477
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10466945
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000082241000008&DestApp=WOS_CPL

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