MISC

2000年

Differential secretion of adrenaline and noradrenaline in response to various secretagogues from bovine chromaffin cells

Clinical and Experimental Pharmacology and Physiology
  • Haruhiro Kuwashima
  • ,
  • Chiaki Matsumura
  • ,
  • Tomohiko Kimura

27
7
開始ページ
494
終了ページ
499
記述言語
英語
掲載種別
DOI
10.1046/j.1440-1681.2000.03284.x

1. Differential secretion of adrenaline (Adr) and noradrenaline (NA) in response to various secretagogues was studied in bovine adrenal chromaffin cells. 2. Acetylcholine (ACh
3-300 μmol/L), 1,1-dimethyl-4-phenyl- piperazinum (DMPP
1-100 μmol/L), high K+ (20-60 mmol/L), calcimycin (1-100 &lt
μmol/L), histamine (0.3-30 μmol/L) and angiotensin (Ang)II (0.3-30 μmol/L) induced the secretion of a 1.3-2-fold greater percentage of NA stores than Adr stores in intact cells. 3. In β-escin-permeabilized cells, Ca2+ (0.1-30 μmol/L) induced a greater secretion of Adr and NA in the presence of MgATP (2mmol/L) than in the absence of MgATP. The percentage of NA secreted was 1.4- and 1.5-fold greater than that of Adr in the presence and absence of MgATP, respectively. 4. The ATP-independent phase of the Ca2+-dependent exocytosis is thought to be associated with the final step that ultimately leads to fusion, while the ATP-dependent phase is thought to be associated with the vesicle priming reaction. Therefore, the preferential secretion of NA in response to ACh, DMPP, high K+, calcimycin, histamine and AngII may be due, at least in part, to the greater effectiveness of Ca2+ in producing exocytosis in NA-containing cells.

リンク情報
DOI
https://doi.org/10.1046/j.1440-1681.2000.03284.x
CiNii Articles
http://ci.nii.ac.jp/naid/80011809938
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10874505

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