MISC

2000年

Lack of Ca2+- and ATP-dependent priming stage in caffeine-induced exocytosis in bovine adrenal chromaffin cells: Comparison with Ca2+

Journal of Autonomic Pharmacology
  • C. Matsumura
  • ,
  • H. Kuwashima
  • ,
  • T. Kimura

20
1
開始ページ
31
終了ページ
36
記述言語
英語
掲載種別
DOI
10.1046/j.1365-2680.2000.00161.x

1. Caffeine (20-40 mM) secreted catecholamines from β-escin-permeabilized bovine adrenal chromaffin cells in the presence or absence of 2 mM MgATP. The caffeine-induced catecholamine secretion in the presence of MgATP was to the same extent as that in the absence of MgATP. 2. Ca2+ (0.1-10 μM) induced a significantly greater secretion of catecholamines in the presence of MgATP than in the absence of MgATP. 3. ML-9 (100 μM) and ML-7 (100 μM), myosin light chain kinase inhibitors, and W-7 (100 μM) and trifluoperazine (TFP
30 μM), calmodulin antagonists, inhibited the Ca2+ -induced catecholamine secretion in the presence of MgATP but not in the absence of MgATP. They did not inhibit the caffeine-induced catecholamine secretion in the presence of MgATP. 4. The ATP-independent phase in Ca2+ -dependent exocytosis is thought to be associated with the final step that ultimately leads to fusion, while the ATP-dependent phase is thought to be associated with a vesicle priming reaction. Therefore, these results suggest that the ATP-requiring priming stage is lacking in the process of caffeine-induced exocytosis in bovine adrenal chromaffin cells.

リンク情報
DOI
https://doi.org/10.1046/j.1365-2680.2000.00161.x
CiNii Articles
http://ci.nii.ac.jp/naid/10010527497
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/11048959

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