MISC

2004年7月

Differential effects of FK506 and cyclosporin A on catecholamine release from bovine adrenal chromaffin cells

Autonomic and Autacoid Pharmacology
  • C. Matsumura
  • ,
  • H. Kuwashima
  • ,
  • T. Kimura

24
3
開始ページ
55
終了ページ
61
記述言語
英語
掲載種別
DOI
10.1111/j.1474-8673.2004.00316.x

1. The effects of the immunosuppressants, tacrolimus (FK506) and cyclosporin A (CsA), on catecholamine (CA) release were examined in cultured bovine adrenal chromaffin cells. 2. In intact cells, FK506 (1-30 μM) inhibited CA release stimulated by acetylcholine (ACh
100 μM), 1,1-dimethyl-4-phenyl-piperazinium (DMPP, 10 μM) or high K+ (40 mM). CsA (1-30 μM) had a little inhibitory effect on the ACh- or DMPP-stimulated CA release, whereas it enhanced the high K+- stimulated CA release. 3. In β-escin-permeabilized cells, FK506 inhibited CA release stimulated by Ca2+ (1 and 10 μM) in the presence and absence of MgATP (2 mM). CsA induced CA release under Ca2+-free condition and enhanced the Ca2+-stimulated CA release in the presence and absence of MgATP. 4. It is known that the Ca2+-dependent exocytosis involves at least two distinct steps, ATP-requiring priming stage and ATP-independent fusion step in adrenal chromaffin cells. Therefore, it is suggested that FK506 inhibits the Ca2+-dependent exocytosis probably at the fusion step whereas CsA induces CA release from bovine adrenal chromaffin cells.

リンク情報
DOI
https://doi.org/10.1111/j.1474-8673.2004.00316.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15541012

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