Papers

Peer-reviewed
5 2015

Influence of different methods of collection from the canine epididymides on post-thaw caudal epididymal sperm quality

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • Tatsuya Hori
  • ,
  • Tetsuya Atago
  • ,
  • Masanori Kobayashi
  • ,
  • Eiichi Kawakami

Volume
77
Number
5
First page
625
Last page
630
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1292/jvms.14-0421
Publisher
JAPAN SOC VET SCI

Canine epididymal sperm was collected from the cauda epididymis using 2 different methods (flushing and mincing) to compare the qualities (the percentage of progressively motile, viable, morphologically abnormal, immature and intact acrosomes) before and after freezing and thawing. No significant difference was noted in the quality of the cauda epididymal sperm immediately after collection and after freezing-thawing between the collection methods, although the mean levels of sperm quality with the flushing method were slightly better than that of the mincing method. The flushing method is simple and free of blood contamination, although the vas deferens was too small to be perfused in only 1 dog, and our results suggest that the flushing method is preferable to the mincing method for collecting sperm from the canine cauda epididymis.

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Link information
DOI
https://doi.org/10.1292/jvms.14-0421
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25649723
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000355368800020&DestApp=WOS_CPL