論文

査読有り
2000年6月

Intrauterine and intravaginal insemination with frozen canine semen using an extender consisting of Orvus ES Paste-supplemented egg yolk tris-fructose citrate

JOURNAL OF VETERINARY MEDICAL SCIENCE
  • T Tsutsui
  • ,
  • M Hase
  • ,
  • A Tanaka
  • ,
  • N Fujimura
  • ,
  • T Hori
  • ,
  • E Kawakami

62
6
開始ページ
603
終了ページ
606
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1292/jvms.62.603
出版者・発行元
JAPAN SOC VET SCI

Our previous report indicated that addition of ON-us ES Paste (OEP) to the extender of frozen canine semen protected acrosomes and maintained sperm motility after thawing. In this study, artificial insemination (AI) using the frozen semen was carried out. The frozen semen was prepared using egg yolk Tris-fructose citrate, and the final concentrations of glycerol and OEP were 7% (v/v) and 0.75% (v/v), respectively. AI was performed during the optimal mating period predicted from the peripheral plasma progesterone level. in intrauterine insemination (IUI), the bitches were laparotomized and 1 x 10(8) spermatozoa were infused into one of the uterine hems. In insemination of non-OEP supplemented semen, 3 x 10(8) spermatozoa were inseminated. In intravaginal insemination (IVI), 10-40 x 10(8) spermatozoa were inseminated. Conception was obtained in nine of 10 bitches (90.0%) that underwent IUI. The number of newborns was from 1 to 7 (mean 3.6 +/- 0.9). The mean ratio of the number of puppies to the number of ovulations in the inseminated uterine horn was 71.8%. The number of puppies did not exceed the number of ovulation in the inseminated uterine hem. Conception using: non-OEP supplemented frozen semen was unsuccessful in all four bitches. In IVI, conception was not obtained in any of the six bitches that received insemination of 10 x 10(8) or 40 x 10(8) spermatozoa, but two of three bitches that received insemination of 20 x 10(8) spermatozoa were fertilized. It was shown that a high conception rate can be obtained by IUI using OEP-supplemented frozen canine semen. Development of a non-surgical method of IUI and a method of freezing canine sperm applicable to IVI is necessary.

Web of Science ® 被引用回数 : 33

リンク情報
DOI
https://doi.org/10.1292/jvms.62.603
Scopus
https://www.scopus.com/record/display.uri?eid=2-s2.0-0034198498&origin=inward
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000088126400008&DestApp=WOS_CPL
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034198498&origin=inward